UBIQUITIN MESSENGER-RNA IS A MAJOR STRESS-INDUCED TRANSCRIPT IN MAMMALIAN-CELLS
UBIQUITIN MESSENGER-RNA IS A MAJOR STRESS-INDUCED TRANSCRIPT IN MAMMALIAN-CELLS
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DOI：
10.1093/nar/17.3.1215
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发表时间：
1989-02-11
影响因子：
14.9
通讯作者：
LAMOREAUX, E
中科院分区：
文献类型：
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作者：
FORNACE, AJ;ALAMO, I;LAMOREAUX, E
在哺乳动物细胞中，泛素mRNA是一种丰富的转录本，它在热休克（HS）和某些其他应激条件下被诱导表达。在中国仓鼠细胞中，2.6 kb和1.7 kb的2种主要泛素转录物被协同诱导，而0.8 kb的次要泛素转录物未被诱导;在人细胞中，2.5 kb UB C和1.0 kb UB B转录物的诱导反应相似。从HS处理的中国仓鼠细胞的cDNA文库中分离的代表性泛素cDNA克隆编码典型的串联重复多聚泛素转录物。该克隆的5“端非翻译序列中只有一部分与已发表的人UB B mRNA的相应区域具有同源性。与5“非翻译序列中与人序列具有同源性的部分互补的寡核苷酸探针以及与无同源性的部分仅与1.7 kb转录物杂交。有协调诱导泛素，热休克蛋白27和热休克蛋白70 mRNA的HS所确定的增加RNA和增加转录。泛素mRNA诱导某些DNA损伤剂，特别是烷基化剂甲基甲烷磺酸盐，或孵育在异亮氨酸缺乏培养基的条件下，HSP mRNA没有。
Ubiquitin mRNA was found to be an abundant transcript which was induced by heat shock (HS), and certain other stresses in mammalian cells. In Chinese hamster cells, the 2 major ubiquitin transcripts of 2.6 kb and 1.7 kb were induced coordinately, while a minor ubiquitin transcript of 0.8 kb was not induced; the response was similar in human cells with induction of the 2.5 kb Ub C and 1.0 kb Ub B transcripts. A representative ubiquitin cDNA clone, isolated from a cDNA library derived from HS-treated Chinese hamster cells, coded for a typical tandem repeat polyubiquitin transcript. Only a portion of the 5'' nontranslated sequence of this clone had homology with the previously published corresponding region in human Ub B mRNA. Oligonucleotide probes complementary to the portion of the 5'' nontranslated sequence with homology to the human sequence and also portions with no homology hybridized only to the 1.7 kb transcript. There was coordinate induction of ubiquitin, HSP27, and HSP70 mRNA by HS as determined by both increased RNA and increased transcription. Ubiquitin mRNA was induced by certain DNA damaging agents, in particular the alkylating agent methylmethane sulfonate, or incubation in isoleucine-deficient medium under conditions where the HSP mRNA were not.