Immunological and biological studies on cholecystokinin in rat brain.
Immunological and biological studies on cholecystokinin in rat brain.
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大鼠脑胆囊收缩素的免疫学和生物学研究。
DOI：
10.1152/ajpendo.1980.239.3.e232
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Walsh,JH
中科院分区：
文献类型：
--
作者：
Lamers,CB;Morley,JE;Poitras,P;Sharp,B;Carlson,HE;Hershman,JM;Walsh,JH
用放射免疫分析法测定了皮质水提液（n = 3）和酸提液（n = 10）中胆囊收缩素样免疫反应性（CCK-LI（42 +/- 9 pmol/g; 4.0 +/- 1.8 pmol/g）、丘脑（4.1 +/- 1.1 pmol/g; 1.0 +/- 0.2 pmol/g）和下丘脑（58 +/- 14 pmol/g; 6.3 +/- 0.7 pmol/g）。Sephadex柱层析显示，95%以上的酸性提取物与CCK 33标准品共洗脱。在水提取物中，80%以上的免疫反应性与CCK 8标准品共洗脱。CCK_（33）和CCK_（8）类物质均能引起离体豚鼠胆囊收缩。L-色氨酸（200 mg/kg）和大剂量吗啡（20 mg/kg）可降低下丘脑和丘脑CCK 33-LI的含量。低剂量吗啡（5 mg/kg）可降低下丘脑CCK 33-LI。结果表明：（1）大鼠大脑皮层、丘脑和下丘脑均有CCK-LI存在;（2）CCK-LI以两种主要的分子形式存在，分别与CCK_（33）和CCK_（8）共存;（3）两种分子形式均具有生物学活性;（4）大鼠大脑中CCK_（33）-LI的浓度受肾上腺素能和阿片类机制的调节。
Cholecystokinin-like immunoreactivity (CCK-LI) was demonstrated by radioimmunoassay in aqueous (n = 3) and acid (n = 10) extracts of cortex (42 +/- 9 pmol/g; 4.0 +/- 1.8 pmol/g), thalamus (4.1 +/- 1.1 pmol/g; 1.0 +/- 0.2 pmol/g), and hypothalamus (58 +/- 14 pmol/g; 6.3 +/- 0.7 pmol/g). Sephadex chromatography revealed that more than 95% of the immunoreactivity in acid extracts coeluted with CCK33 standard. In aqueous extracts more than 80% of immunoreactivity coeluted with CCK8 standard. Both the CCK33- and CCK8-like materials induced contraction of guinea pig gallbladder in vitro. L-Tryptophan (200 mg/kg) and high-dose morphine (20 mg/kg) decreased CCK33-LI concentrations in hypothalamus and thalamus. Low-dose morphine (5 mg/kg) decreased CCK33-LI in hypothalamus. We conclude that 1) CCK-LI is present in cortex, thalamus, and hypothalamus of the rat brain, 2) CCK-LI exists in two predominant molecular forms coeluting with CCK33 and CCK8, 3) both molecular forms are biologically active, and 4) concentrations of rat brain CCK33-LI are modulated by serotonergic and opiate mechanisms.