Feulgen-positive staining of the cell nuclei in fossilized leaf and fruit tissues of the Lower Eocene Myrtaceae
Feulgen-positive staining of the cell nuclei in fossilized leaf and fruit tissues of the Lower Eocene Myrtaceae
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DOI：
10.1111/j.1095-8339.2006.00471.x
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发表时间：
2006-03-01
影响因子：
2.4
通讯作者：
Rodionov, AV
中科院分区：
文献类型：
--
作者：
Ozerov, IA;Zhinkina, NA;Rodionov, AV
用Feulgen反应和Hoechst 33258和4 '，6-diamidino-2-fenilindol（DAPI）两种DNA特异性荧光染料染色，研究了雅库特早始新世桃金娘科（Myrtaceae）、Paramyrtaciphyllum agapovii和Paramyrtaciphyllum pluilocularis的叶片和果实组织中DNA的保存情况。结果表明，染色质结构的植物形成稳定的红紫色复合物与希夫氏品红硫酸试剂原位。这种颜色对DNA是特异性的，特别是对脱氧核糖残基。这意味着这些53-55万年前的植物的细胞核保留了DNA分子的脱氧核糖骨架的一部分。另一方面，用荧光染料DAPI或Hoechst 33258对细胞核没有染色或仅染色非常弱，所述荧光染料DAPI或Hoechst 33258特异性结合双链DNA并且不结合单链DNA或RNA分子。用阿尔新蓝染色的植物化石细胞标本表明，早始新世桃金娘科植物的叶和果实组织的细胞壁中也含有含羧基的多糖。(c)2006伦敦林奈学会，林奈学会植物学杂志，2006年，150，315-321。
The Feulgen reaction and the staining of preparations with two DNA-specific fluorochromes, Hoechst 33258 and 4',6-diamidino-2-fenilindol (DAPI), were used to study the preservation of DNA in the fossilized leaf and fruit tissues of the Lower Eocene Myrtaceae, Paramyrtacicarpus plurilocularis and Paramyrtaciphyllum agapovii collected in Yakutia (Siberia, Russia). It was shown that chromatin structures of the fossilized plants form stable red-purple complexes with the Schiff's fuchsin sulphuric acid reagent in situ. This coloration is specific for DNA, in particular, for the deoxyribose residues. It means that the cell nuclei of these 53-55 Myr old plants preserve a part of the deoxyribose backbone of DNA molecules. On the other hand, there was no, or only a very weak, staining of the cell nucleus with fluorochromes DAPI or Hoechst 33258, which specifically bind to the double-stranded DNA and do not bind to either the single-stranded DNA or RNA molecules. The stainability of fossil plant cell preparations with alcian blue shows that there are also polysaccharides containing carboxyl groups in the cell walls of fossilized leaf and fruit tissues of the Lower Eocene Myrtaceae. (c) 2006 The Linnean Society of London, Botanical Journal of the Linnean Society, 2006, 150, 315-321.