Substance P-, F8Famide-, and A18Famide-like immunoreactivity in the nervus terminalis and retina of the goldfish Carassius auratus
Substance P-, F8Famide-, and A18Famide-like immunoreactivity in the nervus terminalis and retina of the goldfish Carassius auratus
复制标题
金鱼终末神经和视网膜中 P-、F8Famide- 和 A18Famide- 类物质的免疫反应性
作者：
A. Kyle;Bao;T. Magnus;W. Stell
我们使用P物质和其他速激肽的抗血清重新研究了金鱼视网膜中P物质样免疫反应性的发生。大多数抗血清标记的一类以前描述的单层无长突细胞树枝状的第3层的内丛状层。前吸收实验表明，这些无长突细胞含有至少一个速激肽样肽。一种抗P物质的抗血清（INC 353）不仅标记了这些无长突细胞，而且还标记了内丛状层第1层的纤维和视神经中的纤维。这些纤维被确定为retinopetal的终神经的预测，部分原因是共定位标记抗促性腺激素释放激素和FMRFamide的抗血清。前吸收实验表明，P物质的免疫反应物质在终神经不是P物质或任何其他典型的速激肽。用INC 353标记终神经被两种牛FMRFamide样肽F8 Famide和A18 Famide预吸收阻断，这两种肽含有物质P（4-7）样区域。F8 Famide和A18 Famide的抗血清强烈标记终神经和视网膜纤维的神经节。我们建议，终神经的标记物质P和FMRFamide的抗血清发生，因为这些抗原和非速激肽，内源性肽，这是类似于F8 Famide和A18 Famide之间的同源性。
We re-investigated the occurrence of substance P-like immunoreactivity in the retina of the goldfish Carassius auratus using antisera to substance P and other tachykinins. Most antisera labelled a previously described single class of mono-stratified amacrine cells arborizing in layer 3 of the inner plexiform layer. Preabsorption experiments showed that these amacrine cells contained at least one tachykinin-like peptide. One antiserum (INC 353) to substance P labelled not only these amacrine cells but also fibres in layer 1 of the inner plexiform layer and fibres in the optic nerve. These fibres were identified as retinopetal projections of the nervus terminalis, in part because of colocalized labelling with antisera against gonadotropin-releasing hormone and FMRFamide. Preabsorption experiments showed that the substance P-immunoreactive material in the nervus terminalis was not substance P or any other typical tachykinin. Labelling of the nervus terminalis with INC 353 was blocked by preabsorption with two bovine FMRFamide-like peptides, F8Famide and A18Famide, which contain a substance P(4–7)-like region. Antisera to F8Famide and A18Famide strongly labelled ganglia of the nervus terminalis and retinopetal fibres. We suggest that labelling of the nervus terminalis by antisera to substance P and FMRFamide occurs because of homologies between these antigens and a non-tachykinin, endogenous peptide that is similar to F8Famide and A18Famide.
金鱼视网膜中免疫反应物质 P 和神经降压素的表征。
影响因子：
3.4
作者：
Marshak,DW;Carraway,RE;Ferris,CF
通讯作者：
Ferris,CF
编码硬骨鱼促性腺激素释放激素前体及其相关肽的互补 DNA 的表征。
DOI：
10.1210/mend-5-7-931
发表时间：
1991
期刊：
Molecular endocrinology (Baltimore, Md.)
影响因子：
--
作者：
Bond,CT;Francis,RC;Fernald,RD;Adelman,JP
通讯作者：
Adelman,JP