Effects of Dietary Soy Protein Isolate Versus Isoflavones Alone on Poststroke Skilled Ladder Rung Walking and Cortical mRNA Expression Differ in Adult Male Rats.
Effects of Dietary Soy Protein Isolate Versus Isoflavones Alone on Poststroke Skilled Ladder Rung Walking and Cortical mRNA Expression Differ in Adult Male Rats.
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膳食大豆分离蛋白与单独的异黄酮对成年雄性大鼠中风后熟练梯级行走和皮质 mRNA 表达的影响不同。
DOI：
10.1089/jmf.2020.0218
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发表时间：
2022
影响因子：
2.4
通讯作者：
Cheatwood,JosephL
中科院分区：
文献类型：
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作者：
Grisley,ElizabethDawn;Huber,KaleneN;Knapp,AustenN;Butteiger,DustieN;Banz,WilliamJ;MacLean,JamesA;Wallace,DouglasG;Cheatwood,JosephL
饮食中的大豆分离蛋白(SPI)和异黄酮类大豆苷元和染料木素已被证明对中风提供神经保护。然而，机制仍不确定。我们试图确定在以酪酸盐(CAS)为蛋白质来源的饮食中添加异黄酮是否会产生与以前在喂养SPI的大鼠中看到的类似的行为神经保护。此外，我们的目标是表征基线和卒中后mRNAs的表达，这些mRNAs参与了先前发表的可能介导基于大豆的神经保护作用的通路，这些通路可能介导了神经可塑性、氧化应激和炎症的其他标志。成年雄性大鼠饲喂含(1)酪蛋白酸钠(CAS)、(2)CAS+大豆苷元和染料木素(CAS+ISO)或(3)大豆异黄素的半纯饲料2周。对一组大鼠实施安乐死，并收集组织用于实时定量聚合酶链式反应(QPCR)。其余大鼠进行大脑中动脉闭塞以诱发中风。于卒中后第3天采集定量聚合酶链式反应样本。与服用CAS的大鼠相比，服用SPI的大鼠在中风后熟练的阶梯行走任务中犯的错误更少(P<； .05)。饲喂CAS+ISO的大鼠与饲喂CAS或SPI的大鼠无差异。饮食对Syp、PPARG和Ywhe的影响在第0天显著，对Rtn4的表达在第3天有显著影响。我们的结论是，大豆分离蛋白的益处并不完全归因于大豆苷元和染料木素。
Dietary soy protein isolate (SPI) and the isoflavones daidzein and genistein have been shown to provide neuroprotection from stroke. However, the mechanisms remain uncertain. We sought to determine whether the addition of isoflavones to a diet containing caseinate (CAS) as the protein source would induce behavioral neuroprotection similar to that seen previously in rats fed SPI. Furthermore, we aimed to characterize the baseline and poststroke expression of mRNAs involved in pathways previously published as perhaps mediating soy-based neuroprotection from stroke and other markers of neuronal plasticity, oxidative stress, and inflammation. Adult male rats were fed a semipurified diet containing (1) sodium caseinate (CAS), (2) CAS plus daidzein and genistein (CAS+ISO), or (3) SPI for 2 weeks. A subset of rats was euthanized, and tissue was collected for quantitative real-time PCR (qPCR). Remaining rats underwent a middle cerebral artery occlusion to induce a stroke. Samples for qPCR were collected on day 3 poststroke. Rats fed SPI made fewer errors on the skilled ladder rung walking task after stroke compared to rats fed CAS (P< .05). Rats fed CAS+ISO were not different from rats fed CAS or SPI. Significant effects of diet were found at day 0 forSyp,Pparg,and Ywhaeand at day 3 forRtn4expression. We concluded that the benefits of SPI are not solely attributable to daidzein and genistein.