MRI/optical dual-modality imaging of vulnerable atherosclerotic plaque with an osteopontin-targeted probe based on Fe3O4 nanoparticles
MRI/optical dual-modality imaging of vulnerable atherosclerotic plaque with an osteopontin-targeted probe based on Fe3O4 nanoparticles
复制标题
使用基于 Fe3O4 纳米粒子的骨桥蛋白靶向探针对易损动脉粥样硬化斑块进行 MRI/光学双模态成像
DOI：
10.1016/j.biomaterials.2016.10.011
复制
发表时间：
2017-01-01
期刊：
影响因子：
14
通讯作者：
Cao, Feng
中科院分区：
文献类型：
--
作者：
Qiao, Hongyu;Wang, Yabin;Cao, Feng
易损动脉粥样硬化斑块的破裂是急性冠脉综合征管腔血栓形成的主要病理原因。泡沫巨噬细胞是动脉粥样硬化易损性病变的主要成分，骨桥蛋白(OPN)在泡沫巨噬细胞中高表达，OPN可能成为动脉粥样硬化易损性斑块成像的潜在靶点。目前的研究设计了一种OPN特异性MRI/光学双模式探头来检测易损斑块。荧光成像显示，注射Cy5.5-OPN-DMSA-MNPs(COD-MNPs)24 h后，高脂饲料(HFD)组小鼠颈动脉粥样硬化斑块的信号明显高于注射Cy5.5-Ig G-DMSA-MNPs(CID-MNPs)组或注射COD-MNPs的正常饲料组(1.87+/-0.19×10(10)vs 0.74+/-0.04×10(10)，0.73+/-0.03×10(10)p/sec/cm(2)/sr)。P<；0.05)。同时，注射COD-MNPs组大鼠颈动脉斑块注射后24 h，MRI显示颈动脉斑块区域的T-2增强程度明显高于注射CID-MNPs组和正常饮食组(后/前信号比：0.64+/-0.04 vs.0.95+/-0.02，0.98+/-0.01，P<；0.05)。作为一种双模式分子探针，所得到的COD-MNPs结合物在体内非侵入性检测易损动脉粥样硬化斑块方面具有潜在的应用前景。(C)2016爱思唯尔有限公司。保留所有权利。
Rupture of vulnerable atherosclerotic plaque is the major pathological cause of luminal thrombosis in acute coronary syndromes. Since foamy macrophages have been identified as a prominent component in vulnerable atherosclerotic lesions and osteopontin (OPN) is reported to be highly expressed in foamy macrophages, OPN could be a potential target for vulnerable atherosclerotic plaque imaging. The current study designed an OPN-specific MRI/optical dual-modality probe to detect vulnerable plaques. Fluorescence imaging revealed that 24 h after injection of the Cy5.5-OPN-DMSA-MNPs (COD-MNPs), the atherosclerotic plaques in carotid artery exhibited significant higher signals in high fat diet (HFD) fed mice in comparison to the group injected with Cy5.5-IgG-DMSA-MNPs (CID-MNPs) or normal diet fed group injected with COD-MNPs (1.87 +/- 0.19 x 10(10) vs. 0.74 +/- 0.04 x 10(10), 0.73 +/- 0.03 x 10(10) p/sec/cm(2)/sr, P < 0.05). Meanwhile, MRI displayed stronger T-2 contrast enhancement 24 h post-injection at the area of atherosclerotic plaques in the carotid of HFD fed group injected with COD-MNPs than group injected with CID-MNPs or normal diet fed group injected with COD-MNPs (post/pre signal ratio: 0.64 +/- 0.04 vs. 0.95 +/- 0.02, 0.98 +/- 0.01, P < 0.05). As a dual-modality molecular probe, the resulting COD-MNPs conjugates exhibit promising potentials for noninvasive detection of vulnerable atherosclerotic plaque in vivo. (C) 2016 Elsevier Ltd. All rights reserved.