Sonic hedgehog-Gli1 pathway in colorectal adenocarcinomas
Sonic hedgehog-Gli1 pathway in colorectal adenocarcinomas
复制标题
结直肠腺癌中的 Sonic Hedgehog-Gli1 通路
DOI：
10.3748/wjg.v13.i11.1659
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发表时间：
2007-03-21
影响因子：
4.3
通讯作者：
Zhang, Hong-Wei
中科院分区：
文献类型：
--
作者：
Bian, Yue-Hong;Huang, Shu-Hong;Zhang, Hong-Wei
目的：通过分析Shh通路相关分子Shh、Ptch1、hedgehog相互作用蛋白(Hip)、Gli1、Gli3和PDGFRα的表达，探讨Shh通路在结直肠腺癌中的作用。方法：采用RT-PCR、原位杂交和免疫组化方法检测25例结直肠腺癌中Shh的表达。通过原位杂交和免疫组化观察Ptch1的表达。原位杂交分析Hip、Gli1、G13和PDGFRα的表达。结果：结直肠隐窝细胞质中观察到细胞角蛋白AE1/AE3的表达。 Hh 信号通路的成员在结直肠上皮中表达。 Shh表达于发育不良上皮细胞胞浆中，Ptch1、Hip、Gli1主要表达于腺癌恶性隐窝中。相比之下，PDGFR α 在异常隐窝中高表达，在基质中中度表达。结直肠腺癌中未检测到Gli3的表达。结论：这些数据表明Shh-Ptch1-Gli1信号通路可能在结直肠肿瘤的进展中发挥作用。 (c) 2007 年 WJG 出版社。版权所有。
AIM: To determine the role of Sonic hedgehog (Shh) pathway in colorectal adenocarcinomas through analysis of the expression of Shh pathway-related molecules, Shh, Ptch1, hedgehog-interacting protein (Hip), Gli1, Gli3 and PDGFR alpha.METHODS: Expression of Shh in 25 colorectal adenocarcinomas was detected by RT-PCR, in situ hybridization and immunohistochemistry. Expression of Ptch1 was observed by in situ hybridization and immunohistochemistry. Expression of Hip, Gli1, G13 and PDGFR alpha was analyzed by in situ hybridization.RESULTS: Expression of cytokeratin AE1/AE3 was observed in the cytoplasm of colorectal crypts. Members of the Hh signaling pathway were expressed in colorectal epithelium. Shh was expressed in cytoplasm of dysplastic epithelial cells, while expression of Ptch1, Hip and Gli1 were mainly detected in the malignant crypts of adenocarcinomas. In contrast, PDGFR alpha was expressed highly in aberrant crypts and moderately in the stroma. Expression of Gli3 could not be detected in colorectal adenocarcinomas.CONCLUSION:: These data suggest that Shh-Ptch1-Gli1 signaling pathway may play a role in the progression of colorectal tumor. (c) 2007 The WJG Press. All rights reserved.