INHIBITORS OF GROWTH AND AGGREGATION OF CALCIUM OXALATE CRYSTALS IN-VITRO
INHIBITORS OF GROWTH AND AGGREGATION OF CALCIUM OXALATE CRYSTALS IN-VITRO
复制标题
DOI：
10.1016/0009-8981(73)90114-9
复制
发表时间：
1973-01-01
影响因子：
5
通讯作者：
NORDIN, BEC
中科院分区：
文献类型：
--
作者：
ROBERTSO.WG;PEACOCK, M;NORDIN, BEC
建立了一种灵敏的方法来测定结晶剂对草酸钙晶体生长和聚集的影响。在已知的抑制剂中，高电荷阴离子焦磷酸盐和乙烷-1-羟基-1，1-二磷酸盐的浓度可降至0.1-1μ摩尔/L，聚阴离子肝素和硫酸软骨素A的浓度可降至1毫摩尔/L。在尿中尿素的正常浓度范围内，中性分子尿素可引起少量但显着的抑制作用。在较高浓度下，抑制程度急剧增加。在所测试的浓度下，镁离子和亚甲基蓝对晶体的生长和聚集没有影响。这些结果表明，高电荷阴离子和聚阴离子可能对人类反复发生的草酸钙结石有一定的临床治疗价值。
A sensitive method has been developed for measuring the effect of inhibitors of crystallisation on the growth and aggregation of calcium oxalate crystalsin vitro. Of the known inhibitors tested the highly charged anions pyrophosphate and ethane-1-hydroxy-1,1-diphosphonate (EHDP) were effective down to concentrations of 0.1–1 μmole/l and the polyanions heparin and chondroitin sulphate A down to concentrations of 1 nmole/l. Within the normal range of urea concentrations found in urine the neutral molecule urea caused a small but significant degree of inhibition. At higher concentrations the degree of inhibition increased sharply. In the concentrations tested the cations magnesium and methylene blue had no effect on the growth and aggregation of crystals.These findings suggest that highly charged anions and polyanions may have some clinical value as a possible treatment for recurrent calcium oxalate stone-formation in man.