AC Electrothermal Circulatory Pumping Chip for Cell Culture
AC Electrothermal Circulatory Pumping Chip for Cell Culture
复制标题
细胞培养用交流电热循环泵芯片
DOI：
10.1021/acsami.5b08863
复制
发表时间：
2015-12-09
影响因子：
9.5
通讯作者：
Jiang, Hongyuan
中科院分区：
文献类型：
--
作者：
Lang, Qi;Wu, Yanshuang;Jiang, Hongyuan
在这里，我们描述了一种新型的交流电热(ACET)流体循环泵芯片，以克服“芯片上的人”细胞培养系统中液体与组织比的挑战。为了避免焦耳加热和电流对样品池的不利影响，设计了一个矩形微通道，用于泵浦和细胞培养。使用商用热电偶传感器检测抽水区和养殖区的温度值，以检测温度变化。为了产生足够的ACET循环泵速，在泵浦区域使用了30对不对称电极；产生的ACET速度用荧光微粒图像测速仪测量。通过对人胚胎肾细胞(HEK293T)和人结肠癌细胞(5W620)在3V和10 MHz的通电电压下培养72h，证明了该泵浦芯片的优点。细胞生长和增殖良好，表明我们的ACET循环泵芯片在细胞培养和组织工程应用方面具有巨大的潜力。
Herein we describe a novel AC electrothermal (ACET) fluidic circulatory pumping chip to overcome the challenge of fluid-to-tissue ratio for "human-on-a-chip" cell culture systems. To avoid the deleterious effects of Joule heating and electric current on sample cells, a rectangular microchannel was designed with distantly separated regions for pumping and cell culture. Temperature variations were examined using a commercial thermocouple sensor to detect temperature values in both pumping and culture regions. To generate a sufficient ACET circulatory pumping rate, 30 pairs of asymmetrical electrodes were employed in the pumping region; generated ACET velocity was measured by fluorescent microparticle image velocimetry. The benefits of our pumping chip were demonstrated by culturing human embryonic kidney cells (HEK293T) and human colon carcinoma cells (5W620) for 72 h with an energized voltage of 3 V and 10 MHz. Cells grew and proliferated well, implying our ACET circulatory pumping chip has great potential for cell culture and tissue engineering applications.