IMMUNOCYTOCHEMICAL STUDIES ON THE LHRH SYSTEM OF THE JAPANESE QUAIL - INFLUENCE BY PHOTOPERIOD AND ASPECTS OF SEXUAL-DIFFERENTIATION
IMMUNOCYTOCHEMICAL STUDIES ON THE LHRH SYSTEM OF THE JAPANESE QUAIL - INFLUENCE BY PHOTOPERIOD AND ASPECTS OF SEXUAL-DIFFERENTIATION
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DOI：
10.1007/bf00222289
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发表时间：
1988-01-01
影响因子：
3.6
通讯作者：
VIGLIETTIPANZICA, C
中科院分区：
文献类型：
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作者：
FOSTER, RG;PANZICA, GC;VIGLIETTIPANZICA, C
免疫细胞化学被用来确定是否光周期和/或性别有任何影响的模式的促黄体生成激素释放激素（LHRH）系统在日本鹌鹑的大脑。免疫阳性胞体被发现在三个主要领域的大脑：吻侧嗅旁叶，视前区，和隔区。本研究对性成熟鹌鹑经20 h光照：4 h黑暗（20 L ∶ 4 D）24周和生殖系统退化（由20 L ∶ 4 D的光照制度转换为25 L ∶ 16 D的短日照）两种光周期处理后的雌、雄鹌鹑LHRH细胞数量进行了定量分析。双因素方差分析显示，短日照雄鸟的免疫阳性细胞核数（607 ± 134）显著高于长日照雄鸟（291 ± 114）、短日照雌鸟（293 ± 103）和长日照雌鸟（330 ± 92）（p< 0.05）。长日照性成熟鹌鹑（雌雄）正中隆起LHRH免疫反应阳性神经纤维密度和免疫染色强度均高于暴露于8L∶ 16 D 25天的动物。这些结果表明，鹌鹑的LHRH系统受光周期的影响，反映了性别分化。
Immunocytochemistry was used to determine if photoperiod and/or sex have any effect on the pattern of the luteinizing hormone-releasing hormone (LHRH) system in the brain of the Japanese quail. Immunopositive perikarya were found within three major areas of the brain: the rostral paraolfactory lobe, the preoptic, and the septal region. A quantitative analysis of LHRH cell numbers was performed on male and female quail after two photoperiodic treatments: sexually mature birds exposed to 24 weeks of 20 h light: 4 h darkness (20L∶4D), and birds with a regressed reproductive system (induced by transfer from a photoregime of 20L∶4D to 25 short days of 8L∶16D). Two-way analysis of variance showed that short-day males display significantly (p< 0.05) more immunopositive perikarya (607 + 134) than long-day males (291 + 114), short-day females (293 + 103) or long-day females (330 + 92). The density of LHRH-immunoreactive nerve fibres and the intensity of the immunostaining in the median eminence were always greater in long-day sexually mature quail (male and female) than in animals exposed to 25 days of 8L∶16D. These results demonstrate that the LHRH system of the quail is influenced by photoperiod and mirrors sexual differentiation.