Polymorphism of the ovine keratin-associated protein 1-4 gene (KRTAP1-4)
Polymorphism of the ovine keratin-associated protein 1-4 gene (KRTAP1-4)
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DOI：
10.1007/s11033-009-9925-4
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发表时间：
2010-10-01
影响因子：
2.8
通讯作者：
Hickford, Jon G. H.
中科院分区：
文献类型：
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作者：
Gong, Hua;Zhou, Huitong;Hickford, Jon G. H.
角蛋白相关蛋白（KAP）是羊毛纤维的主要结构成分之一，并形成半刚性基质，角蛋白中间丝嵌入其中。KAP基因的变异可能会影响KAP的结构，从而影响羊毛的特性。在这项研究中，我们使用PCR-SSCP分析绵羊KRTAP 1 -4（以前的B2 D），一个基因编码的KAP 1-x家族的成员。在320只绵羊中检测到9种不同的PCR-SSCP图谱。每只绵羊观察到一种或两种模式的组合，这与这些绵羊对该基因的纯合或杂合一致。DNA测序显示，这些模式代表了九种不同的DNA序列。所有这些序列都是独特的，但与已发表的绵羊KRTAP 1 -4序列具有高度同源性，表明这些序列代表KRTAP 1 -4的等位基因变体。共鉴定出14个单核苷酸多态性（SNPs），这些SNPs倾向于聚集在两个区域。在编码区发现的13个SNPs中，9个是非同义SNPs，会导致氨基酸变化。这里检测到的变异可能对KAP 1 -4的结构产生影响，从而影响羊毛性状。
Keratin-associated proteins (KAPs) are one of the main structural components of the wool fibre and form a semi-rigid matrix in which the keratin intermediate filaments are embedded. Variation in the KAP genes may affect the structure of KAPs and hence wool characteristics. In this study, we used PCR-SSCP to analyse ovine KRTAP1-4 (previously B2D), a gene encoding a member of the KAP1-x family. Nine different PCR-SSCP patterns were detected in the 320 sheep that were analysed. Either one or a combination of two patterns was observed for each sheep, which was consistent with these sheep being either homozygous or heterozygous for this gene. DNA sequencing revealed that these patterns represent nine different DNA sequences. All of these sequences were unique, but shared a high homology with the published ovine KRTAP1-4 sequence, suggesting that these sequences represent allelic variants of KRTAP1-4. There were a total of 14 single nucleotide polymorphisms (SNPs) identified and these SNPs tended to be clustered in two regions. Of the 13 SNPs found in the coding region, nine were non-synonymous SNPs and would result in amino acid changes. The variation detected here may have an impact on the structure of KAP1-4 and hence affect wool traits.