MORPHOLOGICAL ANALYSIS OF THE TUBEROMAMMILLARY NUCLEUS IN THE RAT-BRAIN - DELINEATION OF SUBGROUPS WITH ANTIBODY AGAINST L-HISTIDINE DECARBOXYLASE AS A MARKER
MORPHOLOGICAL ANALYSIS OF THE TUBEROMAMMILLARY NUCLEUS IN THE RAT-BRAIN - DELINEATION OF SUBGROUPS WITH ANTIBODY AGAINST L-HISTIDINE DECARBOXYLASE AS A MARKER
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DOI：
10.1002/cne.902630102
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发表时间：
1987-09-01
影响因子：
2.5
通讯作者：
KOHLER, C
中科院分区：
文献类型：
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作者：
ERICSON, H;WATANABE, T;KOHLER, C
用L-组氨酸脱羧酶（HD）特异性抗体，结合免疫组织化学和逆行荧光示踪技术，研究了成年雄性大鼠下丘脑后部组胺能神经元的形态、分布和投射。大细胞神经元，位于两侧的乳头状隐窝和接近的腹侧表面的头和尾的乳头体，被发现含有HD免疫反应（HD-i）。除了这些大细胞神经元，大量的小型和中型的神经元进行免疫染色，作为桥接HD-1细胞群的各种大小的细胞链。HD-1细胞在额叶，矢状和水平切片的详细映射显示，这些神经元组成一个连续的细胞群，定义为结节乳头核（TM）。这个核可以分为几个亚组。因此，位于乳头状窝两侧的大约600个HD-1神经元组成了TM（TMM）的内侧亚组。TM的腹侧亚组（TMV）由位于脑腹侧表面、乳头体的吻侧（TMVr）和尾侧（TMVc）的约1，500个神经元组成。与TMV相比，TMM含有更大比例的小细胞神经元。约100个HD-i细胞分散在下丘脑外侧区、下丘脑后区、穿区、乳头体上核和下丘脑背内侧核内。这些细胞统称为TM的弥散部分（TMdiff）。TMM和TMV之间的形态差异并没有信号这些亚组的传出连接的差异。因此，将荧光示踪剂固蓝单次注射到大脑的不同区域，包括脊髓，导致HD-1神经元的逆行标记，其分布在整个TM中，没有可辨别的地形图案。 更具体地说，TM的每个亚组贡献了支配或穿过大量脑区域的投射，包括嗅球、海马、尾状核、下丘脑的室旁核和视上核、小脑、顶盖、髓质和脊髓。HD-i神经元的广泛投射与从位于TM周围的细胞群中的非HD-i神经元观察到的更具体的投射形成对比。两者合计，这些发现表明，HD-i细胞的后下丘脑构成一个主要的核，TM，这个核可以被细分为三个组件，其中每个都有弥漫性的投射在整个神经轴。
With an antibody specific for L-histidine decarboxylase (HD) in combination with immunohistochemical techniques and retrograde fluorescent tracing, the morphology, distribution, and projections of the histaminergic neurons of the posterior hypothalamus were studied in the adult male rat. Magnocellular neurons, situated on both sides of the mammillary recess and close to the ventral surface of the brain rostral and caudal to the mammillary bodies, were found to contain HD-immunoreactivity (HD-i). In addition to these magnocellular neurons, a substantial number of small and medium-sized neurons were immunostained, as were strands of cells of all sizes bridging the HD-i cell groups. A detailed mapping of the HD-i cells in frontal, sagittal, and horizontal sections showed that these neurons make up one continuous cell group, defined as the tuberomammillary nucleus (TM). This nucleus can be divided into several subgroups. Thus, approximately 600 HD-i neurons situated on each side of the mammillary recess compose the medial subgroup of the TM (TMM). The ventral subgroup of the TM (TMV) consists of some 1,500 neurons situated at the ventral surface of the brain, rostral (TMVr) and caudal (TMVc) to the mammillary bodies. The TMM contains a greater proportion of parvicellular neurons compared to the TMV. About 100 HD-i cells are scattered within the lateral hypothalamic area, the posterior hypothalamic region, the perfornical area, the supramammillary nucleus, and the dorsomedial hypothalamic nucleus. These cells are collectively named the diffuse part of the TM (TMdiff). The morphological differences between the TMM and the TMV did not signal differences in the efferent connections of these subgroups. Thus, single injections of the fluorescent tracer Fast Blue into different regions of the brain, including the spinal cord, resulted in retrograde labeling of HD-i neurons, which were distributed throughout the TM with no discernible topographic pattern. More specifically, each subgroup of the TM contributed projections innervating or passing through a large number of brain regions, including the olfactory bulb, hippocampus, caudate nucleus, paraventricular and supraoptic nuclei of the hypothalamus, cerebellum, tectum, medulla, and spinal cord. The widespread projections of the HD-i neurons contrasted to the more specific projections observed from non HD-i neurons in cell groups situated around the TM. Taken together, these findings suggest that the HD-i cells of the posterior hypothalamus constitute one major nucleus, the TM, and that this nucleus may be subdivided into three components, each of which has diffuse projections throughout the neuraxis.