1H(C) and 1H(N) total NOE correlations in a single 3D NMR experiment. 15N and 13C time-sharing in t1 and t2 dimensions for simultaneous data acquisition.
1H(C) and 1H(N) total NOE correlations in a single 3D NMR experiment. 15N and 13C time-sharing in t1 and t2 dimensions for simultaneous data acquisition.
复制标题
单个 3D NMR 实验中的 1H(C) 和 1H(N) 总 NOE 相关性。
DOI：
10.1023/a:1025407905478
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发表时间：
2003
影响因子：
2.7
通讯作者：
Gao,Xiaolian
中科院分区：
文献类型：
--
作者：
Xia,Youlin;Yee,Adelinda;Arrowsmith,CherylH;Gao,Xiaolian
Simultaneous data acquisition in time-sharing (TS) multi-dimensional NMR experiments has been shown an effective means to reduce experimental time, and thus to accelerate structure determination of proteins. This has been accomplished by spin evolution time-sharing of the X and Y heteronuclei, such as15N and13C, in one of the time dimensions. In this work, we report a new 3D TS experiment, which allows simultaneous13C and15N spin labeling coherence in botht1andt2dimensions to give four NOESY spectra in a single 3D experiment. These spectra represent total NOE correlations between1HNand1HCresonances. This strategy of double time-sharing (2TS) results in an overall four-fold reduction in experimental time compared with its conventional counterpart. This 3D 2TS CN-CN-H HSQC-NOESY-HSQC pulse sequence also demonstrates improvements in water suppression,15N spectral resolution and sensitivity, which were developed based on 2D TS CN-H HSQC and 3D TS H-CN-H NOESY-HSQC experiments. Combining the 3D TS and the 3D 2TS NOESY experiments, NOE assignment ambiguities and errors are considerably reduced. These results will be useful for rapid protein structure determination to complement the effort of discerning the functions of diverse genomic proteins.
