Amino acid sequence of the catalytic subunit of bovine type II adenosine cyclic 3',5'-phosphate dependent protein kinase.
Amino acid sequence of the catalytic subunit of bovine type II adenosine cyclic 3',5'-phosphate dependent protein kinase.
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牛 II 型腺苷环状 3,5-磷酸依赖性蛋白激酶催化亚基的氨基酸序列。
DOI：
10.1021/bi00284a025
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发表时间：
1983
期刊：
影响因子：
2.9
通讯作者：
Titani,K
中科院分区：
文献类型：
--
作者：
Shoji,S;Ericsson,LH;Walsh,KA;Fischer,EH;Titani,K
Shozo Shoji,** Lowell H. Ericsson, Kenneth A. Walsh, Edmond H. Fischer和Koiti Titani*摘要：描述了牛心肌腺苷环3',5'-磷酸依赖蛋白激酶催化亚基的350个残基氨基酸序列。该蛋白分子量为40862，包含一个阻断NH2末端的iv -十四烷基（肉豆蔻酰基）和位于苏氨酸-197和丝氨酸-338的磷酸基团。用溴化氰裂解7个甲硫基键，得到8个初级肽。
Shozo Shoji,** Lowell H. Ericsson, Kenneth A. Walsh, Edmond H. Fischer, and Koiti Titani* abstract: The 3 50-residue amino acid sequence of the catalytic subunit of bovine cardiac muscle adenosine cyclic 3', 5'-phosphate dependent protein kinase is described. The protein has a molecular weight of 40 862, which includes an iV-tetradecanoyl (myristyl) group blocking the NH2 terminus and phosphate groups at threonine-197 and serine-338. Seven methionyl bonds in theS-carboxymethylated protein were cleaved with cyanogen bromide to yield eight primary peptides.