Mapping a double flower phenotype-associated gene DcAP2L in Dianthus chinensis.
Mapping a double flower phenotype-associated gene DcAP2L in Dianthus chinensis.
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石竹双花表型相关基因 DcAP2L 的定位。
DOI：
10.1093/jxb/erz558
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发表时间：
2020
影响因子：
6.9
通讯作者：
Fu Xiaopeng
中科院分区：
文献类型：
--
作者：
Wang Qijian;Zhang Xiaoni;Lin Shengnan;Yang Shaozong;Yan Xiuli;Bendahmane Mohammed;Bao Manzhu;Fu Xiaopeng
重瓣花是影响许多开花植物观赏价值的重要育种性状。为了更好地了解中国石竹重瓣花形成的遗传机制，我们利用140个单瓣花基因型与重瓣花基因型杂交的F2代构建了高密度遗传图谱。使用双酶切限制性位点相关DNA测序（ddRAD-seq）与2353个单核苷酸多态性（SNP）构建连锁图谱。对12个园艺性状进行了QTL定位分析，确定了9个主效QTL。其中2个主效QTL分别对花瓣数变异的贡献率为20.7%和78.1%。进行混合分离RNA-seq（BSR-seq）以准确地搜索与双花性状相关的候选基因。利用石竹参考基因组进行QTL定位和BSR-seq分析的结果表明，石竹A类花器官同一性基因APETALA 2（DcAP 2L）miR 172切割位点的SNP突变通过调控DcAG基因的表达而导致了石竹重瓣花的形成。
The double flower is a highly important breeding trait that affects the ornamental value in many flowering plants. To get a better understanding of the genetic mechanism of double flower formation inDianthus chinensis, we have constructed a high-density genetic map using 140 F2progenies derived from a cross between a single flower genotype and a double flower genotype. The linkage map was constructed using double-digest restriction site-associated DNA sequencing (ddRAD-seq) with 2353 single nucleotide polymorphisms (SNPs). Quantitative trait locus (QTL) mapping analysis was conducted for 12 horticultural traits, and major QTLs were identified for nine of the 12 traits. Among them, two major QTLs accounted for 20.7% and 78.1% of the total petal number variation, respectively. Bulked segregant RNA-seq (BSR-seq) was performed to search accurately for candidate genes associated with the double flower trait. Integrative analysis of QTL mapping and BSR-seq analysis using the reference genome ofDianthus caryophyllussuggested that an SNP mutation in the miR172 cleavage site of the A-class flower organ identity geneAPETALA2(DcAP2L) is responsible for double flower formation inDianthusthrough regulating the expression ofDcAGgenes.