Vasopressin Promoter Transgenic and Vasopressin Gene-Edited Ascidian, Ciona intestinalis Type A (Ciona robusta): Innervation, Gene Expression Profiles, and Phenotypes.
Vasopressin Promoter Transgenic and Vasopressin Gene-Edited Ascidian, Ciona intestinalis Type A (Ciona robusta): Innervation, Gene Expression Profiles, and Phenotypes.
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DOI：
10.3389/fendo.2021.668564
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发表时间：
2021
影响因子：
5.2
通讯作者：
Satake H
中科院分区：
文献类型：
--
作者：
Kawada T;Shiraishi A;Matsubara S;Hozumi A;Horie T;Sasakura Y;Satake H
催产素（OT）和血管加压素（VP）超家族神经肽不仅分布于脊椎动物，而且分布于多种无脊椎动物。然而，无脊椎动物的VPergic神经支配从未被记录。在海鞘，玻璃海鞘A型（Ciona robusta），OT/VP超家族肽被确定，和玻璃海鞘加压素（CiVP）诱导卵母细胞成熟和排卵。在本研究中，我们的特点的神经支配和表型的遗传修饰的玻璃海鞘：CiVP启动子金星转基因和CiVP突变体。CiVP启动子-Venus转基因玻璃海鞘表明CiVP基因在脑神经节和多种神经中高表达。在年轻的CiVP启动子-Venus转基因海鞘的卵巢中也检测到荧光，表明CiVP基因也在年轻海鞘的卵巢中暂时表达。此外，一个显着减少后卵黄发生（阶段III）的卵泡中观察到的CiVP突变体的卵巢，而前卵黄发生（阶段I）和卵黄发生（阶段II）的卵泡增加的突变体卵巢，与野生型玻璃海鞘。基因表达谱显示，各种基因的表达，包括与卵泡生长相关的基因，在CiVP突变体的卵巢中发生了改变。总之，这些结果表明，CiVP，主要作为一种神经肽，在多种生物学功能中起着关键作用，包括通过调节多种基因的表达来促进早期卵泡的生长。这是第一份描述VP基因启动子转基因和VP基因编辑的无脊椎动物及其基因表达谱和表型的报告。
Oxytocin (OT) and vasopressin (VP) superfamily neuropeptides are distributed in not only vertebrates but also diverse invertebrates. However, no VPergic innervation of invertebrates has ever been documented. In the ascidian, Ciona intestinalis Type A (Ciona robusta), an OT/VP superfamily peptide was identified, and the Ciona vasopressin (CiVP) induces oocyte maturation and ovulation. In the present study, we characterize the innervation and phenotypes of genetically modified Ciona: CiVP promoter-Venus transgenic and CiVP mutants. CiVP promoter-Venus transgenic Ciona demonstrated that CiVP gene was highly expressed in the cerebral ganglion and several nerves. Fluorescence was also detected in the ovary of young CiVP promoter-Venus transgenic ascidians, suggesting that the CiVP gene is also expressed temporarily in the ovary of young ascidians. Furthermore, a marked decrease of post-vitellogenic (stage III) follicles was observed in the ovary of CiVP mutants, whereas pre-vitellogenic (stage I) and vitellogenic (stage II) follicles were increased in the mutant ovary, compared with that of wildtype Ciona. Gene expression profiles showed that the expression of various genes, including genes related to ovarian follicle growth, was altered in the ovary of CiVP mutants. Altogether, these results indicated that CiVP, mainly as a neuropeptide, plays pivotal roles in diverse biological functions, including growth of early-stage ovarian follicles via regulation of the expression of a wide variety of genes. This is the first report describing a VP gene promoter-transgenic and VP gene-edited invertebrate and also on its gene expression profiles and phenotypes.
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影响因子：
3.8
作者：
Aikins, Michael J.;Schooley, David A.;Park, Yoonseong
通讯作者：
Park, Yoonseong
影响因子：
4.6
作者：
Sakuma, Tetsushi;Ochiai, Hiroshi;Kaneko, Takehito;Mashimo, Tomoji;Tokumasu, Daisuke;Sakane, Yuto;Suzuki, Ken-ichi;Miyamoto, Tatsuo;Sakamoto, Naoaki;Matsuura, Shinya;Yamamoto, Takashi
通讯作者：
Yamamoto, Takashi
影响因子：
5.2
作者：
Kawada T;Shiraishi A;Aoyama M;Satake H
通讯作者：
Satake H
DOI：
10.1126/science.1226201
发表时间：
2012-10-26
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Garrison JL;Macosko EZ;Bernstein S;Pokala N;Albrecht DR;Bargmann CI
通讯作者：
Bargmann CI
影响因子：
2.8
作者：
Elphick, Maurice R.;Rowe, Matthew L.
通讯作者：
Rowe, Matthew L.