Overexpression and characterization of a new organic solvent-tolerant esterase derived from soil metagenomic DNA
Overexpression and characterization of a new organic solvent-tolerant esterase derived from soil metagenomic DNA
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源自土壤宏基因组 DNA 的新型耐有机溶剂酯酶的过表达和表征
DOI：
10.1016/j.biortech.2011.10.087
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发表时间：
2012-07-01
影响因子：
11.4
通讯作者：
Wang, Qiuyan
中科院分区：
文献类型：
--
作者：
Jin, Peng;Pei, Xiaolin;Wang, Qiuyan
本研究从土壤宏基因组文库中分离到一种酯酶，命名为EstC23。在T7启动子的控制下，该蛋白可在大肠杆菌中过表达，从而表达出占细胞总蛋白含量30%的活性可溶性蛋白。EstC23在高达50% （v/v）的苯和烷烃（高对数P溶剂）中表现出显著的稳定性。在含有50%苯或烷烃的环境中培养7天后，酶的活性保持了2-3倍的提高。纯化后的酶还能裂解位阻叔醇酯。这些结果表明，EstC23具有在工业过程中使用的潜力。(C) 2011 Elsevier Ltd.版权所有。
In this study, an esterase, designated EstC23, was isolated from a soil metagenomic library. The protein was amenable to overexpression in Escherichia coli under control of the T7 promoter, resulting in expression of the active, soluble protein that constituted 30% of the total cell protein content. This enzyme showed optimal activity at 40 degrees C and retained about 50% maximal activity at 5-10 degrees C. EstC23 showed remarkable stability in up to 50% (v/v) benzene and alkanes (high log P solvents). When incubated for 7 days in the presence of 50% benzene or alkanes, the enzyme maintained its 2-3 fold elevated activity. The purified enzyme also cleaved sterically hindered esters of tertiary alcohols. These results indicate that EstC23 has potential for use in industrial processes. (C) 2011 Elsevier Ltd. All rights reserved.