Full genome sequences of human immunodeficiency virus type 1 subtypes G and A/G intersubtype recombinants
Full genome sequences of human immunodeficiency virus type 1 subtypes G and A/G intersubtype recombinants
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DOI：
10.1006/viro.1998.9211
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发表时间：
1998-07-20
期刊：
影响因子：
3.7
通讯作者：
McCutchan, FE
中科院分区：
文献类型：
--
作者：
Carr, JK;Salminen, MO;McCutchan, FE
在HIV-I分离株中已鉴定出多种基因亚型和亚型间重组株。从中非发现的菌株多样性最大，在那里发现了亚型和重组形式的混合物。然而，许多HIV-1亚型和重组体的特征在于部分而不是全长基因组测序。在这里，我们报告了前两个几乎全长的基因组序列从HIV-I亚型G，分离在瑞典和芬兰，但起源于刚果和肯尼亚，并从两个吉布提分离共享A/G重组结构的尼日利亚分离，IbNG。通过与其他亚型的参考序列比较，似乎G亚型毒株在很大程度上是非重组的，而吉布提毒株显示来自A亚型和G亚型的交替片段。在吉布提病毒gp 41蛋白质的胞质结构域中，E、G和IbNG毒株形成一个单独的簇，与亚型A分开，模糊了这些特定片段的亚型起源。在现有技术的分辨率范围内，吉布提菌株的结构与IbNG的结构相同，首次确定了这种重组体在非洲的地理分布。IbNG样菌株的地理分布表明，像E亚型重组体一样，这些重组体应该被赋予一个特定的名称，以便于将来的识别和跟踪;建议命名为“IbNG亚型”。(C)北京：科学出版社.
Multiple genetic subtypes and intersubtype recombinant strains have been identified among isolates of HIV-I. The greatest diversity of strains has been recovered from Central Africa, where mixtures of subtypes and recombinant forms have been recovered. However, many of the HIV-l subtypes and recombinants have been characterized by partial rather than full-length genome sequencing. Here we report the first two virtually full-length genome sequences from HIV-I subtype G, isolated in Sweden and Finland but originating in Congo and Kenya, and from two Djibouti isolates sharing the A/G recombinant structure of Nigerian isolate, IbNG. By comparison with reference sequences of other subtypes, it appears that the subtype G strains are largely nonrecombinant, while the Djibouti strains show alternating segments from subtypes A and G. In the cytoplasmic domain of the gp41 protein of the Djibouti viruses the E, G, and IbNG strains form a single cluster, separate from subtype A, clouding the subtype origin of these particular segments. Within the resolution of current technology, the structure of the Djibouti strains is identical to that of IbNG, establishing for the first time the geographic spread of this recombinant in Africa. The geographic spread of the IbNG-like strains suggests that, like the subtype E recombinants, these should be given a specific name to facilitate future identification and tracking; the name "lbNG subtype" is proposed. (C) 1998 Academic Press.