Effect of non-surgical periodontal treatment on short chain fatty acid levels in gingival crevicular fluid of patients with generalized aggressive periodontitis
Effect of non-surgical periodontal treatment on short chain fatty acid levels in gingival crevicular fluid of patients with generalized aggressive periodontitis
复制标题
非手术牙周治疗对广泛性侵袭性牙周炎患者龈沟液短链脂肪酸水平的影响
DOI：
10.1111/jre.12137
复制
发表时间：
2014-10-01
影响因子：
3.5
通讯作者：
Feng, X.
中科院分区：
文献类型：
--
作者：
Lu, R.;Meng, H.;Feng, X.
背景和目的：短链脂肪酸（SCFA）在牙周疾病中发挥重要作用。然而，侵袭性牙周炎患者龈沟液中 SCFA 的浓度尚不清楚。本干预研究的目的是调查非手术牙周治疗对广泛性侵袭性牙周炎 (G-AgP) 患者龈沟液中 SCFA 水平的影响，并分析有或没有检测到推定牙周病原体部位的 SCFA 浓度。 材料和方法：从 G-AgP 患者的滤纸条上收集 80 份龈沟液样本（每个受试者 4 份）（n = 20；平均年龄 24.5 岁），非手术牙周治疗前和治疗后 2 周、2、4 和 6 个月。从牙周健康对照（n = 20；平均年龄 26.2 岁）中收集了 80 份龈沟液样本（每个受试者 4 份）。采用高性能毛细管电泳法测定龈沟液样品上清液中甲酸、琥珀酸、乙酸、乳酸、丙酸、丁酸和异戊酸的浓度。采用聚合酶链式反应扩增对相同预处理的龈沟液标本沉淀物中的牙龈卟啉单胞菌、齿垢密螺旋体、放线菌聚集菌、中间普氏菌和具核梭杆菌进行分析。结果：G-AgP患者非手术牙周治疗后6个月内的临床参数明显改善。处理后甲酸浓度显着升高；与阴性位点相比，P. gingivalis-、T. denticola-、P. intermedia-或 F. nucleatum 阳性位点的甲酸水平较低。治疗后乙酸、丙酸和丁酸浓度显着降低，治疗后2周达到最低水平，但治疗后2个月后有上升趋势，且P.gingivalis、T. denticola、P. intermedia-或F. nucleatum阳性部位的3种SCFA水平显着高于阴性部位。结论：非手术牙周治疗导致乙酸显着降低。 G-AgP 患者龈沟液中酸、丙酸、丁酸水平和甲酸水平增加，并伴有临床参数的改善。 G-AgP患者龈沟液中甲酸含量明显较低，而乙酸、丙酸和丁酸含量较高，与牙周病原菌感染一致。
Background and Objective: Short chain fatty acids (SCFAs) play important roles in periodontal diseases. However, the concentrations of SCFAs in gingival crevicular fluid of patients with aggressive periodontitis are not known. The aim of this intervention study was to investigate the influences of non-surgical periodontal therapy on levels of SCFAs in the gingival crevicular fluid of patients with generalized aggressive periodontitis (G-AgP), and analyze the concentrations of SCFAs in sites with or without the detected putative periodontal pathogens.Material and Methods: Eighty gingival crevicular fluid samples (four per subject) were collected on filter paper strips from patients with G-AgP (n = 20; mean age 24.5 years), before and at 2 wk, 2, 4 and 6 mo after non-surgical periodontal treatment. Eighty gingival crevicular fluid samples (four per subject) were collected from periodontally healthy controls (n = 20; mean age 26.2 years). Concentrations of formic acid, succinic acid, acetic acid, lactic acid, propionic acid, butyric acid and isovaleric acid from the supernatant of gingival crevicular fluid samples were measured by high performance capillary electrophoresis. Porphyromonas gingivalis, Treponema denticola, Aggregatibacter actinomycetemcomitans, Prevotella intermedia and Fusobacterium nucleatum from the precipitate of the same pretreatment samples of gingival crevicular fluids were analyzed by polymerase chain reaction amplification.Results: The clinical parameters of patients with G-AgP during the 6 mo after non-surgical periodontal treatment were improved remarkably. The formic acid concentration increased significantly after treatment; the level of formic acid was lower in the P. gingivalis-, T. denticola-, P. intermedia-or F. nucleatum-positive sites compared with the negative sites. The concentrations of acetic acid, propionic acid and butyric acid reduced significantly after treatment and reached the lowest level at 2 wk post-treatment, although showed a tendency to increase after 2 mo post-treatment, and the three SCFA levels were significantly higher in P. gingivalis-, T. denticola-, P. intermedia-or F. nucleatum-positive sites compared with those in the negative sites.Conclusion: Non-surgical periodontal treatment resulted in a significant decrease of acetic acid, propionic acid, butyric acid levels and increase of formic acid level in gingival crevicular fluids in patients with G-AgP, accompanied by improvement in clinical parameters. A marked lower level of formic acid, as well as higher levels of acetic acid, propionic acid and butyric acid in gingival crevicular fluid of patients with G-AgP was consistent with periodontal pathogen infection.