Detection of antibody against lymphocystis disease virus in Japanese flounder by enzyme linked immunosorbent assay
Detection of antibody against lymphocystis disease virus in Japanese flounder by enzyme linked immunosorbent assay
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酶联免疫吸附法检测牙鲆淋巴细胞病病毒抗体
DOI：
10.3147/jsfp.33.207
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发表时间：
1998
期刊：
影响因子：
0.6
通讯作者：
Y. Ezura
中科院分区：
文献类型：
--
作者：
H. Nishida;M. Yoshimizu;Y. Ezura
采用酶联免疫吸附法（ELISA）检测日本比目鱼（parichthys olivaceus）抗淋巴囊肿病病毒（LCDV）抗体。以纯化的牙鲆LCDV作为ELISA的捕获抗原。采用表面健康鱼、lcd病鱼和lcd康复鱼的血清监测了日本比目鱼对LCDV的免疫反应。三组的ELISA吸光度有明显差异。利用吸光度高的血清，建立最佳的酶联免疫吸附试验条件。给看似健康的鱼注射灭活的LCDV。注射后1 ~ 3个月，用LCDV建立的酶联免疫吸附试验（ELISA）检测抗LCDV抗体，在此期间抗体滴度升高。
Enzyme linked immunosorbent assay (ELISA) was applied to detect the antibody of Japanese flounder (Paralichthys olivaceus) against lymphocystis disease virus (LCDV). Purified Japanese flounder LCDV was used as a capture antigen of ELISA. Monitoring the immune response of Japanese flounder to LCDV was done using the sera obtained from apparently healthy, LCD-diseased and LCD-recovered fish. ELISA absorbances of these three groups were clearly different. Using the serum with high ELISA absorbance, the optimum ELISA condition was set up. Apparently healthy fish were injected with inactivated LCDV. One to three months after the injection, the antibody against LCDV was detected by ELISA established for LCDV and their ELISA antibody titer increased during that period.