Identification of quantitative trait loci for cadmium tolerance and accumulation in wheat
Identification of quantitative trait loci for cadmium tolerance and accumulation in wheat
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小麦耐镉和积累数量性状位点的鉴定
DOI：
10.1007/s11738-011-0818-5
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发表时间：
2012-01-01
影响因子：
2.6
通讯作者：
Cao, Weixing
中科院分区：
文献类型：
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作者：
Ci, Dunwei;Jiang, Dong;Cao, Weixing
小麦(Triticum aestivum L.)耐镉积累的数量性状基因座(QTL)用103个来自CH×Sh杂交组合的重组自交系在萌发期和苗期进行鉴定。测定了种子萌发、生长和生理特性。计算了Cd胁迫下植物相对于对照的耐镉指数(TI)。测定了根部和地上部的Cd浓度，计算了Cd的积累量和转移量。上述性状的表型变异在重组自交系之间呈连续分布格局。共检测到26个QTL(其中16个为对照和Cd胁迫下的性状，8个为耐镉性状，2个为根Cd积累性状)。这26个QTL可以解释7.97-60.16%的表型变异。14个QTL为正(加性效应来自于CH)，其余12个为负(加性效应由Sh贡献)。在小麦染色体的同一区域未检测到QTL。结果表明，控制耐镉性状的遗传机制是相互独立的。因此，在本研究中，小麦的耐镉和积累特性是独立的性状。
Quantitative trait loci (QTL) for Cadmium (Cd) tolerance and accumulation in wheat (Triticum aestivum L.) were identified, using 103 recombinant inbred lines (RILs) derived from a cross of Ch x Sh at germination and seedling stages. The traits of germination, growth and physiology were measured. Cd tolerance indexes (TI) were calculated for plants under Cd stress relative to control conditions. Cd concentrations in both root and shoot were determined and the amount of Cd accumulation and translocation calculated. The phenotypic variation of the above traits showed a continuous distribution pattern among the RILs. Twenty-six QTLs were detected, (16 of which were designated for the traits under the control and Cd stress, 8 for Cd tolerance and 2 for root Cd accumulation). These 26 QTLs individually could explain 7.97-60.16% of the phenotypic variation. Fourteen QTLs were positive (with the additive effects coming from Ch) while the remaining 12 QTLs were negative (with the additive effects contributed by Sh). No QTL were detected in the same region on the chromosomes of wheat. The results indicated that genetic mechanisms controlling the traits of Cd tolerance were independent from each other. Therefore, in this study, the properties of Cd tolerance and accumulation showed to be independent traits in wheat.