Follicular atresia in the prepubertal spiny mouse (Acomys cahirinus) ovary
Follicular atresia in the prepubertal spiny mouse (Acomys cahirinus) ovary
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DOI：
10.1007/s10495-011-0626-9
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发表时间：
2011-10-01
期刊：
影响因子：
7.2
通讯作者：
Gawron, Antoni
中科院分区：
文献类型：
--
作者：
Hulas-Stasiak, Monika;Gawron, Antoni
本研究旨在确定新生儿和青春期前多刺小鼠的卵泡闭锁。我们使用细胞凋亡（TUNEL 反应、活性 caspase-3）和自噬（Lamp1）的经典标记物分析了卵泡丢失的过程。在所有卵泡类型的死亡卵母细胞中，尤其是原始卵母细胞和初级卵母细胞中，观察到许多小的透明液泡和自噬体以及强烈的 Lamp1 染色。仅在大次级卵泡和窦卵泡的颗粒细胞中检测到活性 caspase 3 和 TUNEL 反应。细胞凋亡和自噬标记物的表达在青春期前也发生变化。 Western blot分析表明，女性在出生时，自噬介导的卵泡闭锁率增加，而细胞凋亡是出生后连续几天卵巢闭锁的主要形式。基于这些观察，我们得出结论，细胞凋亡和自噬与卵泡闭锁有关，并且这些过程是细胞和发育阶段特异性的。
This study was designed to determine follicular atresia in the newborn and the prepubertal spiny mouse. We analyzed the processes of follicle loss using classical markers of apoptosis (TUNEL reaction, active caspase-3) and autophagy (Lamp1). Numerous small clear vacuoles and autophagosomes as well as strong Lamp1 staining were observed in dying oocytes of all follicle types, especially of the primordial and primary ones. Active caspase 3 and the TUNEL reaction were detected only in the granulosa cells of large secondary and antral follicles. The expression of apoptosis and autophagy markers was also changing during the prepubertal period. Western blot analysis indicated that at the moment of birth, females undergo an increased rate of follicular atresia mediated by autophagy, while apoptosis is the dominant form of ovarian atresia in consecutive postnatal days. On the basis of these observations, we concluded that apoptosis and autophagy are involved in follicular atresia and these processes are cell and developmental stage-specific.