Enhanced production of microbial metabolites in the presence of dimethyl sulfoxide
Enhanced production of microbial metabolites in the presence of dimethyl sulfoxide
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DOI：
10.7164/antibiotics.53.1145
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发表时间：
2000-10-01
影响因子：
3.3
通讯作者：
Davies, J
中科院分区：
文献类型：
--
作者：
Chen, GH;Wang, GYS;Davies, J
生长的细菌菌株：在低浓度二甲基亚砜（DMSO）的存在下，次级代谢产物的产生表现出显着的定性和定量变化。这种效应在多种生物合成家族中得到证实，包括氯霉素（分支酸盐）、硫链丝菌素（肽）和四环霉素（聚酮化合物），以及天然和重组的链霉菌菌株;在产芽孢杆菌（如B）中也观察到类似的效应。circulans.抗生素产量的增加不是这些生物体生长速率变化的结果，因为在有和没有DMSO的培养基中生物质的产量相似（高达3%）。我们认为，添加化合物，如DMSO提供了一种检查微生物的全部生物合成潜力的方法，并可能用于促进次级代谢产物的产生。DMSO的作用方式尚不清楚，但在研究的病例中，它可能在翻译水平起作用。
Bacterial strains grown: in the presence of low concentrations of dimethyl sulfoxide (DMSO) exhibit significant qualitative and quantitative alterations in the production of secondary metabolites. This effect was confirmed for a variety of biosynthetic families, including chloramphenicol (chorismate), thiostrepton (peptide) and tetracenomycin (polyketide), and for natural and recombinant strains of streptomycetes; a similar effect was seen with antibiotic-producing bacilli such as B. circulans. Increase in antibiotic production was not the result of a change in the growth rate of these organisms, since yields of biomass were similar in media with and without DMSO (up to 3%). We suggest that the addition of compounds such as DMSO provides a means of examining the full biosynthetic potential of microbes and might be used to promote secondary metabolite production. The mode of action of DMSO is not known, but in the cases studied it may act at the level of translation.