Protective effect of hydroxytyrosol and its metabolite homovanillic alcohol on H2O2 induced lipid peroxidation in renal tubular epithelial cells
Protective effect of hydroxytyrosol and its metabolite homovanillic alcohol on H2O2 induced lipid peroxidation in renal tubular epithelial cells
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DOI：
10.1016/j.fct.2008.05.037
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发表时间：
2008-09-01
影响因子：
4.3
通讯作者：
Dessi, M. Assunta
中科院分区：
文献类型：
--
作者：
Deiana, Monica;Incani, Alessandra;Dessi, M. Assunta
我们研究了羟基酪醇（HT），3，4-二羟基苯基乙醇，高香草酸醇（HVA），4-羟基-3-甲氧基-苯基乙醇，抑制过氧化氢诱导的猪肾上皮细胞株LLC-PK 1的氧化损伤的能力，研究了过氧化氢油特异性细胞膜脂质靶点，不饱和脂肪酸和胆固醇的影响。暴露于H2 O2诱导的MDA水平的显着增加，连同膜结构的破坏，与不饱和脂肪酸，胆固醇和α-生育酚的损失，和脂肪酸氢过氧化物和7-酮基胆固醇的形成。HT预处理保护肾细胞免受氧化损伤：膜脂质水平得到保护，氧化产物检测不出。HVA具有相当的活性，因此HT和HVA都能够防止肾细胞中在肾小管细胞损伤中起核心作用的脂质过氧化过程。(C)2008爱思唯尔有限公司保留所有权利。
We investigated the capacity of hydroxytyrosol (HT), 3,4-dihydroxyphenylethanol, and homovanillic alcohol (HVA), 4-hydroxy-3-methoxy-phenylethanol, to inhibit H2O2 induced oxidative damage in LLC-PK1, a porcine kidney epithelial cell line, studying the effect of H2O2 Oil specific cell membrane lipid targets, unsaturated fatty acids and cholesterol. Exposure to H2O2 induced a significant increase of the level of MDA together with a disruption of the membrane structure, with the loss of unsaturated fatty acids, cholesterol and of alpha-tocopherol, and the formation of fatty acids hydroperoxides and 7-ketocholesterol. Pretreatment with HT protected renal cells from oxidative damage: the level of membrane lipids was preserved and there was no significant detection of oxidation products. HVA exerted a comparable activity, thus both HT and HVA were able to prevent in renal cells the lipid peroxidation process that plays a central role in tubular cell injury. (C) 2008 Elsevier Ltd. All rights reserved.