Neuropeptidergic Signaling in the American Lobster Homarus americanus: New Insights from High-Throughput Nucleotide Sequencing.
Neuropeptidergic Signaling in the American Lobster Homarus americanus: New Insights from High-Throughput Nucleotide Sequencing.
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DOI：
10.1371/journal.pone.0145964
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Dickinson PS
中科院分区：
文献类型：
--
作者：
Christie AE;Chi M;Lameyer TJ;Pascual MG;Shea DN;Stanhope ME;Schulz DJ;Dickinson PS
肽是用于神经化学通讯的最大和最多样化的一类分子，在生理和行为的基本所有方面的控制中发挥关键作用。美洲龙虾，Homarus americanus，是一种具有商业和生物医学重要性的甲壳类动物;龙虾的生长和繁殖受神经肽能控制，龙虾神经系统的一部分作为理解节律运动行为（包括肽能神经调节）的一般原则的模型。虽然已经从H.虽然已经在细胞/系统水平上研究了美洲龙虾的神经肽能信号传导，并且已经在细胞/系统水平上研究了一些的作用，但是目前对龙虾中神经肽能信号传导的分子组分知之甚少。在这里，一个H产生美洲神经转录组并挖掘编码推定肽前体和受体的序列;鉴定了35种前体和41种受体编码转录物。我们从推导的前体蛋白中预测了194种不同的神经肽，包括脂肪动力学调节因子corazonin样肽、allatostatin A、allatostatin C、囊体激素、CCHamide、corazonin、甲壳动物心脏活性肽、甲壳动物高血糖激素（CHH）、CHH受体相关肽、利尿激素31、利尿激素44、羽化激素、FLRF酰胺、GSEFL酰胺、胰岛素样肽、intocin、leucokinin、肌抑制素、neuroparsin、神经肽F、orcokinin、色素分散激素、proctolin、pyrokinin、SIFamide、sulfakinin和速激肽相关肽家族。虽然一些预测的肽是已知的H。americanus同种型，大多数是新的鉴定，是现存龙虾神经肽组的两倍以上。推导的受体蛋白是H. americanus神经肽受体，包括前面提到的大多数肽组的受体，以及蜕皮触发激素、红色素浓缩激素和短神经肽F的受体。对于大多数肽家族，鉴定出多种受体。这些数据代表了对H. americanus，并将作为未来的神经肽能控制的基因为基础的研究在龙虾。
Peptides are the largest and most diverse class of molecules used for neurochemical communication, playing key roles in the control of essentially all aspects of physiology and behavior. The American lobster, Homarus americanus, is a crustacean of commercial and biomedical importance; lobster growth and reproduction are under neuropeptidergic control, and portions of the lobster nervous system serve as models for understanding the general principles underlying rhythmic motor behavior (including peptidergic neuromodulation). While a number of neuropeptides have been identified from H. americanus, and the effects of some have been investigated at the cellular/systems levels, little is currently known about the molecular components of neuropeptidergic signaling in the lobster. Here, a H. americanus neural transcriptome was generated and mined for sequences encoding putative peptide precursors and receptors; 35 precursor- and 41 receptor-encoding transcripts were identified. We predicted 194 distinct neuropeptides from the deduced precursor proteins, including members of the adipokinetic hormone-corazonin-like peptide, allatostatin A, allatostatin C, bursicon, CCHamide, corazonin, crustacean cardioactive peptide, crustacean hyperglycemic hormone (CHH), CHH precursor-related peptide, diuretic hormone 31, diuretic hormone 44, eclosion hormone, FLRFamide, GSEFLamide, insulin-like peptide, intocin, leucokinin, myosuppressin, neuroparsin, neuropeptide F, orcokinin, pigment dispersing hormone, proctolin, pyrokinin, SIFamide, sulfakinin and tachykinin-related peptide families. While some of the predicted peptides are known H. americanus isoforms, most are novel identifications, more than doubling the extant lobster neuropeptidome. The deduced receptor proteins are the first descriptions of H. americanus neuropeptide receptors, and include ones for most of the peptide groups mentioned earlier, as well as those for ecdysis-triggering hormone, red pigment concentrating hormone and short neuropeptide F. Multiple receptors were identified for most peptide families. These data represent the most complete description of the molecular underpinnings of peptidergic signaling in H. americanus, and will serve as a foundation for future gene-based studies of neuropeptidergic control in the lobster.