Methionine-directed fabrication of gold nanoclusters with yellow fluorescent emission for Cu2+ sensing
Methionine-directed fabrication of gold nanoclusters with yellow fluorescent emission for Cu2+ sensing
复制标题
蛋氨酸引导制备具有黄色荧光发射的金纳米簇，用于 Cu2 传感
DOI：
10.1016/j.bios.2014.10.071
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发表时间：
2015-03-15
影响因子：
12.6
通讯作者：
Chen, Wei
中科院分区：
文献类型：
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作者：
Deng, Hao-Hua;Zhang, Ling-Na;Chen, Wei
近年来，荧光金纳米团簇由于其独特的物理、电学和光学性质，在物理、化学、材料科学和生物科学等领域受到了广泛的关注。本文首次报道了用蛋氨酸作为还原剂和稳定剂合成水溶性单分散AuNCs的方法。该合成过程绿色且简单，并且所得到的由蛋氨酸覆盖的AuNCs （Met-AuNCs）将与生物有机体具有生物相容性。通过紫外可见吸收、光致发光、x射线光电子能谱（XPS）和傅里叶变换红外光谱（FTIR）表征了所制备的Met-AuNCs的化学组成和光学性质。met - aunc具有许多吸引人的特点，包括强烈的黄色荧光（在530 nm处最大发射），长荧光寿命（181 ns和1651 ns），高胶体稳定性（pH-，温度-，盐-和时间稳定性），以及大斯托克位移（110 nm），作为生命科学和环境研究的新型分析工具具有很大的希望。此外，所合成的Met-AuNCs可以作为荧光探针，通过荧光猝灭选择性检测Cu2+。Cu2+的检出限为7.9 nM，在50 nM ~ 81.1 m范围内呈线性响应。此外，新构建的探针可以简单快速地检测土壤中Cu2+的浓度，结果表明它对实际样品中铜的测定具有很大的可行性。(C) 2014 Elsevier B.V.版权所有
In the past few years, fluorescent gold nanoclusters (AuNCs) have gained much attention in many areas of physics, chemistry, materials science, and biosciences due to their unique physical, electrical, and optical properties. Herein, we reported for the first time the synthesis of water soluble, monodispersed AuNCs by using methionine both as a reductant and a stabilizer. The synthetic process is green and simple, and the resulting AuNCs capped by methionine (Met-AuNCs) would be biocompatible with bioorganisms. UV visible absorption, photoluminescence, X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared (FTIR) were carried out to demonstrate the chemical composition and optical properties of the as-prepared Met-AuNCs. The Met-AuNCs possess many attractive features including intense yellow fluorescence (emission maximum at 530 nm), a long fluorescence lifetime (181 ns and 1651 ns), high colloidal stability (pH-, temperature-, salt- and time-stability), and a large Stoke's shift (110 nm), holding great promise as late-model analytical tools for life science and environmental studies. Moreover, the as-synthesized Met-AuNCs can serve as an efficient fluorescent probe for selective detection of Cu2+ by fluorescence quenching. The limit of detection for Cu2+ was determined to be 7.9 nM and linear response over the Cu2+ concentrations range from 50 nM to 81.1.M. Furthermore, the new-constructed probe allows simple and rapid detection of the concentrations of Cu2+ in soil, with results demonstrating its great feasibility for the determination of copper in real samples. (C) 2014 Elsevier B.V. All rights reserved.