Role for 5-HT and ACh in submucosal reflexes mediating colonic secretion.
Role for 5-HT and ACh in submucosal reflexes mediating colonic secretion.
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5-HT 和 ACh 在介导结肠分泌的粘膜下反射中的作用。
DOI：
10.1152/ajpgi.1995.269.3.g346
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Cooke,HJ
中科院分区：
文献类型：
--
作者：
Sidhu,M;Cooke,HJ
在豚鼠远端结肠节段上研究了粘膜刺激激活的神经反射通路。用刷子以1/s的速度敲击全层粘膜表面或肌肉剥离段引起短路电流（Isc）增加，其持续时间和幅度取决于敲击次数。通过氯化物分泌抑制剂和河豚毒素，粘膜中风引起的Isc增加几乎被消除。阿托品、伦扎必利和N-乙酰基-5-羟色胺-5-羟色氨酸酰胺可降低反应，但美加明、酮色林、托烷司琼或SDZ-205-557不会降低反应。5-羟色胺和5-羟吲哒平的粘液应用增加了Isc，而河豚毒素和伦扎必利则减弱了Isc。结果表明，粘膜中风引起的氯化物分泌激活神经反射途径，利用胆碱能受体，5-HT 1 P类，以及粘膜下神经丛内的毒蕈碱受体。
Neural reflex pathways activated in response to mucosal stroking were investigated in segments of distal colon from guinea pigs. Stroking the mucosal surface of whole thickness or muscle-stripped segments with a brush at 1/s evoked an increase in short-circuit current (Isc) whose duration and amplitude were dependent on the number of strokes. The increase in Isc induced by mucosal stroking was virtually abolished by inhibitors of chloride secretion and by tetrodotoxin. The response was reduced by atropine, renzapride, and N-acetyl-5-hydroxytryptophyl-5-hydroxytryptophan amide but not by mecamylamine, ketanserin, tropisetron, or SDZ-205-557. Mucosal application of 5-hydroxytryptamine and 5-hydroxyindalpine increased Isc, which was attenuated by tetrodotoxin and renzapride. The results suggest that mucosal stroking evokes chloride secretion by activating neural reflex pathways utilizing serotoninergic receptors, of the 5-HT1P class, as well as muscarinic receptors within the submucosal plexus.