Dinoflagellate expressed indicate massive transfer to the nuclear genome sequence tag data of chloroplast genes
Dinoflagellate expressed indicate massive transfer to the nuclear genome sequence tag data of chloroplast genes
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DOI：
10.1078/1434461000165
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发表时间：
2004-03-01
期刊：
影响因子：
2.5
通讯作者：
Delwiche, CF
中科院分区：
文献类型：
--
作者：
Bachvaroff, TR;Concepcion, GT;Delwiche, CF
甲藻的多甲藻素色素质体知之甚少，部分原因是缺乏这些内共生细胞器的分子数据。为了鉴定将携带关于多甲藻素型甲藻质体的生物学和其进化历史的信息的额外基因序列，对从从Lingulodinium polyedrum（1012个非冗余序列）和Amphidinium carterae（2143）构建的cDNA文库中任意选择的序列进行分析。在这两个文库中，共鉴定出118个独特的质体相关序列，其中30个（大部分来自A. carterae），这些基因编码在红紫菜的质体基因组中。这些序列可能代表真正的核基因，并建议有大量的基因转移从质体到核基因组的甲藻。这些数据支持了多甲苷型质体具有最小基因组的假设，并提供了与多甲苷型质体中存在未鉴定的典型基因组的假设相矛盾的数据。还鉴定了可能直接从红藻内共生体的核基因组转移的序列，以及其他与泡藻不同的序列。这些数据的初步报告在威斯康星州麦迪逊举行的2002年植物学会议上提出。
The peridinin-pigmented plastids of dinoflagellates are very poorly understood, in part because of the paucity of molecular data available from these endosymbiotic organelles. To identify additional gene sequences that would carry information about the biology of the peridinin-type dinoflagellate plastid and its evolutionary history, an analysis was undertaken of arbitrarily selected sequences from cDNA libraries constructed from Lingulodinium polyedrum (1012 non-redundant sequences) and Amphidinium carterae (2143). Among the two libraries 118 unique plastid-associated sequences were identified, including 30 (most from A. carterae) that are encoded in the plastid genome of the red alga Porphyra. These sequences probably represent bona fide nuclear genes, and suggest that there has been massive transfer of genes from the plastid to the nuclear genome in dinoflagellates. These data support the hypothesis that the peridinin-type plastid has a minimal genome, and provide data that contradict the hypothesis that there is an unidentified canonical genome in the peridinin-type plastid. Sequences were also identified that were probably transferred directly from the nuclear genome of the red algal endosymbiont, as well as others that are distinctive to the Alveolata. A preliminary report of these data was presented at the Botany 2002 meeting in Madison, WI.