INHIBITION OF MALIGNANT-CELL GROWTH BY KETONE-BODIES
INHIBITION OF MALIGNANT-CELL GROWTH BY KETONE-BODIES
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DOI：
10.1038/icb.1979.54
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发表时间：
1979-01-01
期刊：
影响因子：
--
通讯作者：
CONYERS, RAJ
中科院分区：
文献类型：
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作者：
MAGEE, BA;POTEZNY, N;CONYERS, RAJ
本文研究了酮体对培养的转化淋巴母细胞（[人伯基特淋巴瘤]Raji细胞）生长的影响。细胞生长受到抑制，这种作用是可逆的，无毒的，并且与D- β的浓度成正比。总葡萄糖利用率和总乳酸产量与细胞增殖抑制成比例降低。D度量。-羟基丁酸不被细胞代谢。其他糖酵解抑制剂和D- β的化学类似物。-羟基丁酸不抑制细胞生长或被证明对细胞有太大的毒性。D度量。-羟基丁酸抑制兔肾RK13、[人宫颈癌]HeLa、小鼠黑色素瘤B16、成纤维细胞和胰蛋白酶分散的人甲状腺和牛睾丸细胞的生长。体内饮食诱导的酮症使C57BL/6小鼠肺中的B16黑色素瘤沉积数量减少了2/3。讨论了这些结果在癌症恶病质临床治疗中的意义。
The effect of ketone bodies on the growth, in culture, of transformed lymphoblasts ([human Burkitt''s lymphoma] Raji cells) was investigated. Cell growth was inhibited and this effect was reversible, non-toxic and proportional to the concentration of D-.beta.-hydroxybutyrate up to 20 mM. The total glucose utilization and the total lactate production were reduced in proportion to the inhibition of cell proliferation. D-.beta.-hydroxybutyrate was not metabolized by the cells. Other glycolytic inhibitors and chemical analogues of D-.beta.-hydroxybutyrate did not inhibit or proved to be too toxic for cell growth. D-.beta.-hydroxybutyrate inhibited the growth of rabbit kidney RK13, [human cervical carcinoma] HeLa, mouse melanoma B16, fibroblast and trypsin-dispersed human thyroid and beef testis cells. In vivo dietary-induced ketosis reduced the number of B16 melanoma deposits in the lungs of C57BL/6 mice by 2/3. The significance of these results in the clinical management of cancer cachexia is discussed.