Dietary soy isoflavones differentially regulate expression of the lipid-metabolic genes in different white adipose tissues of the female Bama mini-pigs
Dietary soy isoflavones differentially regulate expression of the lipid-metabolic genes in different white adipose tissues of the female Bama mini-pigs
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DOI：
10.1016/j.bbrc.2015.04.006
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发表时间：
2015-05-22
影响因子：
3.1
通讯作者：
Yin, Yulong
中科院分区：
文献类型：
--
作者：
Jiang, Guoli;Li, Lili;Yin, Yulong
大豆异黄酮已被证明影响脂质代谢，但其潜在的分子机制尚未完全了解。本研究以雌性巴马迷你猪为模型，研究了大豆异黄酮对不同白色脂肪组织脂质代谢及相关基因表达的影响。试验选用35日龄雌性巴马香迷你猪，分别饲喂基础饲粮（对照、Con）和在基础饲粮中添加大豆异黄酮（250、500、1250 mg/kg饲粮）120 d。结果表明，大豆异黄酮对体重没有影响，但降低了背侧皮下脂肪组织（DSA）质量，增加了腹部皮下脂肪组织（ASA）和肾周脂肪组织（PRA）质量。此外，大豆异黄酮降低了DSA中脂肪生成基因的表达，增加了脂溶基因的表达，而在ASA和PRA中则相反。此外，大豆异黄酮降低了DSA中脂蛋白脂肪酶的表达，而上调了ASA和PRA中脂蛋白脂肪酶的表达。大豆异黄酮可上调DSA中雌激素受体（er）的表达，下调ASA和PRA中雌激素受体（er）的表达。本研究结果提示大豆异黄酮对巴马猪白色脂肪组织脂质代谢的影响具有位点特异性，可能通过ppar和er调控基因表达介导。(C) 2015爱思唯尔公司版权所有。
Soy isoflavones have been shown to affect lipid metabolism, however the underlying molecular mechanism(s) have not yet been fully understood. The present study, using female Bama mini-pig as a model, examined the effects of soy isoflavones on lipid metabolism and involved gene expression in different white adipose tissues. Female Bama Xiang mini-pigs of 35 days old were fed a basal diet (control, Con), or basal diet supplemented with increasing amounts of soy isoflavones (250, 500, or 1250 mg/kg diet) for 120 days. The results showed that soy isoflavones did not affect the body weight, but decreased the dorsal subcutaneous adipose tissue (DSA) mass and increased the mass of abdominal subcutaneous adipose tissue (ASA) and perirenal adipose tissue (PRA). Besides, soy isoflavones decreased the expression of lipogenic genes and increased the expression of lipolytic genes in DSA, while the opposite effects were observed in ASA and PRA. In addition, the expression of lipoprotein lipase was down regulated in DSA while up regulated in ASA and PRA by soy isoflavones. Moreover, the expression of estrogen receptors (ERs) was up regulated in DSA, and down regulated in ASA and PRA by soy isoflavones. Our results suggest that soy isoflavones affected the lipid metabolism in white adipose tissues of Bama mini-pigs in a site-specific manner, which might be mediated through PPARs and ERs regulated gene expression. (C) 2015 Elsevier Inc. All rights reserved.