Lactococcus termiticola sp. nov., isolated from the gut of higher termite Nasutitermes takasagoensis

Lactococcus termiticola sp. nov., isolated from the gut of higher termite Nasutitermes takasagoensis
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白蚁乳球菌 sp.

DOI:
10.1099/ijsem.0.003068
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发表时间:
2018
期刊:
IJSEM
影响因子:
--
通讯作者:
and M. Ohkuma
and M. Ohkuma
中科院分区:
--
文献类型:
--
作者:
S. Noda;M. Sakamoto;C. Aihara;M. Yuki;M. Katsuhara;and M. Ohkuma

文献摘要

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对从食木高等白蚁(asutitermes takasagoensis)肠道中分离得到的一株乳酸菌NtB2T进行了遗传和表型鉴定。菌株ntb2t与乳酸乳球菌属有亲缘关系。tructaeJCM 31125从褐鳟中分离得到,16S rRNA基因和rpob基因序列相似性分别为93.2%和81.0%。此外,利用两两平均核苷酸同源性分析和基因组-基因组距离计算器对菌株NtB2TandL进行基因组比较。lactisubsp。tructaeJCM 31125t的值分别为81.0和23.2%。菌株ntb2t3产生的主要细胞脂肪酸为c18.1 ω9c和c16.0 ω。菌株ntb2t细胞壁肽聚糖类型为A3α, Lys-Gly-Ser-Ala2。根据所提供的数据,该分离物代表了乳球菌属的一种新种,其名称为乳球菌。11月被提议。型应变为NtB2T(=JCM 32569T=DSM 107259T)。
A strain of lactic acid bacteria, designated NtB2T, isolated from the gut of the wood-feeding higher termiteNasutitermes takasagoensis, was characterized genetically and phenotypically. Strain NtB2Twas related toLactococcus lactisubsp.tructaeJCM 31125Tisolated from brown trout, showing 93.2 and 81.0 % similarity in 16S rRNA gene andrpoBgene sequences, respectively. Furthermore, genomic comparisons using pairwise average nucleotide identity analysis and the Genome-to-Genome Distance Calculator between strain NtB2TandL. lactisubsp.tructaeJCM 31125Tgave values of 81.0 and 23.2 %, respectively. Major cellular fatty acids produced by strain NtB2Twere C18 : 1ω9cand C16 : 0. The cell-wall peptidoglycan type of strain NtB2Twas A3α, Lys–Gly–Ser–Ala2. Based on the data presented, the isolate represents a novel species of the genusLactococcus, for which the nameLactococcus termiticolasp. nov. is proposed. The type strain is NtB2T(=JCM 32569T=DSM 107259T).