Inorganic polyphosphate abets silencing of a sub-telomeric gene cluster in fission yeast.
Inorganic polyphosphate abets silencing of a sub-telomeric gene cluster in fission yeast.
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DOI：
10.17912/micropub.biology.000744
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发表时间：
2023
影响因子：
--
通讯作者：
Shuman, Stewart
中科院分区：
文献类型：
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作者：
Sanchez, Ana M;Garg, Angad;Schwer, Beate;Shuman, Stewart
无机聚磷酸盐是一种普遍存在的聚合物，在细胞和有机体生理学中具有多种作用。尽管有证据表明核聚磷酸盐存在，但其对真核生物转录调控的影响尚不清楚。对缺乏多磷酸盐的裂殖酵母细胞进行转录分析（通过删除 Vtc4/Vtc2 多磷酸聚合酶复合物的催化亚基 Vtc4），引起位于 I 号染色体右亚端粒臂内的四个蛋白质编码基因的去抑制，已知该基因被 TORC2 复合物转录沉默。这些基因在 vtc2 Δ 细胞和表达聚合酶死亡 Vtc4 的细胞中同样去抑制，这表明多磷酸盐合成是抑制这些亚端粒基因所必需的。
Inorganic polyphosphate is a ubiquitous polymer with myriad roles in cell and organismal physiology. Whereas there is evidence for nuclear polyphosphate, its impact on transcriptional regulation in eukaryotes is unkown. Transcriptional profiling of fission yeast cells lacking polyphosphate (via deletion of the catalytic subunit Vtc4 of the Vtc4/Vtc2 polyphosphate polymerase complex) elicited de-repression of four protein-coding genes located within the right sub-telomeric arm of chromosome I that is known to be transcriptionally silenced by the TORC2 complex. These genes were equally de-repressed in vtc2 ∆ cells and in cells expressing polymerase-dead Vtc4, signifying that polyphosphate synthesis is required for repression of these sub-telomeric genes.