Chloroplast genome sequences from total DNA for plant identification
Chloroplast genome sequences from total DNA for plant identification
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DOI：
10.1111/j.1467-7652.2010.00558.x
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发表时间：
2011-04-01
影响因子：
13.8
通讯作者：
Henry, Robert J.
中科院分区：
文献类型：
--
作者：
Nock, Catherine J.;Waters, Daniel L. E.;Henry, Robert J.
叶绿体DNA序列数据是植物鉴定或条形码化以及在植物物种之间建立遗传关系的通用工具。不同的叶绿体基因座已被用于植物中近距离和远距离的进化距离，没有一个单一的基因座已被确定，可以区分所有的植物物种。DNA测序技术的进步为更大规模的基因组比较提供了新的具有成本效益的选择。然而，叶绿体序列的通用PCR扩增或纯叶绿体组分的分离是不平凡的。我们现在提出从总DNA的大规模并行测序（MPS）分析叶绿体基因组序列作为植物条形码的简单且具有成本效益的选择，并分析植物关系以指导生物技术的基因发现。本文报道了五种禾本科植物叶绿体基因组全序列。这些数据准确地建立了物种之间的系统发育关系，纠正了已发表的水稻序列中的一个明显错误。叶绿体基因组可能是植物的难以捉摸的单基因座DNA条形码。
Chloroplast DNA sequence data are a versatile tool for plant identification or barcoding and establishing genetic relationships among plant species. Different chloroplast loci have been utilized for use at close and distant evolutionary distances in plants, and no single locus has been identified that can distinguish between all plant species. Advances in DNA sequencing technology are providing new cost-effective options for genome comparisons on a much larger scale. Universal PCR amplification of chloroplast sequences or isolation of pure chloroplast fractions, however, are nontrivial. We now propose the analysis of chloroplast genome sequences from massively parallel sequencing (MPS) of total DNA as a simple and cost-effective option for plant barcoding, and analysis of plant relationships to guide gene discovery for biotechnology. We present chloroplast genome sequences of five grass species derived from MPS of total DNA. These data accurately established the phylogenetic relationships between the species, correcting an apparent error in the published rice sequence. The chloroplast genome may be the elusive single-locus DNA barcode for plants.