Repeated electroacupuncture preconditioning attenuates matrix metalloproteinase-9 expression and activity after focal cerebral ischemia in rats
Repeated electroacupuncture preconditioning attenuates matrix metalloproteinase-9 expression and activity after focal cerebral ischemia in rats
复制标题
重复电针预处理减弱大鼠局灶性脑缺血后基质金属蛋白酶9的表达和活性
DOI：
10.1179/174313209x393960
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发表时间：
2009-10-01
影响因子：
1.9
通讯作者：
Xiong, Li-Ze
中科院分区：
文献类型：
--
作者：
Dong, Hui;Fan, Yan-Hong;Xiong, Li-Ze
目的：研究电针预处理对缺血后脑半球血脑屏障（BBB）完整性及基质金属蛋白酶-9 （MMP-9）表达的影响。方法：采用大脑中动脉闭塞法（MCAO）诱导大鼠局灶性脑缺血。动物随机分为正常组、假手术组、MCAO组和EA组。EA组给予大鼠百会穴（gv20）电针刺激30分钟/天，连续5天。末次治疗24小时后行MCAO。MCAO后24小时测定脑含水量和血脑屏障通透性。MMP-9在MCAO后6、12和24小时的表达和活性测定。结果：EA组大鼠脑缺血半球含水量（81.45 +/- 1.09%）低于MCAO组（83.98 +/- 1.30%）
Objective: This study investigates the effects of electroacupuncture (EA) preconditioning on blood-brain barrier (BBB) integrity and matrix metalloproteinase-9 (MMP-9) expression in subsequent ischemic hemisphere.Methods: Focal cerebral ischemia was induced by middle cerebral artery occlusion (MCAO) in rats. Animals were randomly divided into four groups: normal, sham-operated, MCAO and EA groups. In EA group, rats received electroacupuncture stimuli at the Baihui acupoint (GV 20) 30 minutes/day for 5 days. Twenty-four hours after last treatment, the MCAO was performed. The brain water content and BBB permeability were measured 24 hours after MCAO. MMP-9 expression and activity were measured at 6, 12 and 24 hours after MCAO.Results: The results showed that the brain water content of ischemic hemisphere was lower in EA group (81.45 +/- 1.09%) compared with MCAO group (83.98 +/- 1.30%; p