PC12 ADHESION AND NEURITE FORMATION ON SELECTED SUBSTRATES ARE INHIBITED BY SOME GLYCOSAMINOGLYCANS AND A FIBRONECTIN-DERIVED TETRAPEPTIDE
PC12 ADHESION AND NEURITE FORMATION ON SELECTED SUBSTRATES ARE INHIBITED BY SOME GLYCOSAMINOGLYCANS AND A FIBRONECTIN-DERIVED TETRAPEPTIDE
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DOI：
10.1016/0014-4827(86)90340-x
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发表时间：
1986-02-01
影响因子：
3.7
通讯作者：
WARREN, SL
中科院分区：
文献类型：
--
作者：
AKESON, R;WARREN, SL
测试了添加的可溶性糖胺聚糖 (GAG) 对培养的 PC12 嗜铬细胞瘤细胞在多种基质上的粘附和神经突形成的影响。与未涂覆的培养皿或组织培养塑料相比，PC12 细胞在涂有纤连蛋白、层粘连蛋白、聚-L-赖氨酸或 conA 的培养皿上粘附得更快。添加的硫酸葡聚糖显着抑制对聚-L-赖氨酸、纤连蛋白和层粘连蛋白包被的培养皿的粘附，并在较小程度上抑制肝素，但不被硫酸软骨素抑制。 PC12 对纤连蛋白的粘附也可以被假定的纤连蛋白细胞结合四肽 L-精氨酰-甘氨酰-L-天冬氨酰-L-丝氨酸完全抑制（Pierschbacher，MD 和 Ruoslahti，E，Nature 309（1984）30）[25]。该四肽与肝素或硫酸葡聚糖（但不是硫酸软骨素或透明质酸）的组合的抑制作用是相加的。神经生长因子 (NGF) 预处理增加了 PC12 细胞粘附于所有基质的百分比，并减少了 GAG 对粘附的抑制。先前用 NGF 处理以诱导形态分化的 PC12 细胞在铺在所有四种基质上时将迅速重新延伸神经突。在纤连蛋白和聚-L-赖氨酸包被的培养皿上，这种神经突生长会被添加的肝素和硫酸葡聚糖抑制，而在层粘连蛋白上则不会。低浓度的纤连蛋白四肽也抑制纤连蛋白包被的培养皿上的神经突形成。总之，PC12粘附和神经突形成可以被某些底物（包括纤连蛋白）上的硫酸化GAG抑制，但不能抑制其他底物，这表明这些细胞至少具有两种独立的分子粘附机制。
The effects of added soluble glycosaminoglycans (GAGs) on adhesion and neurite formation by cultured PC12 pheochromocytoma cells on several substrates were tested. PC12 cells adhere more rapidly to Petri plastic coated with fibronectin, laminin, poly-L-lysine, or conA, than to either uncoated Petri plastic or tissue culture plastic. Adhesion to poly-L-lysine, fibronectin- and laminin-coated dishes was significantly inhibited by added dextran sulfate and to a lesser extent heparin-but not by chondroitin sulfate. PC12 adhesion to fibronectin could also be totally inhibited by the putative fibronectin cell binding tetrapeptide L-arginyl-glycyl-L-aspartyl-L-serine (Pierschbacher, M D and Ruoslahti, E, Nature 309 (1984) 30) [25]. The inhibitory effects of combinations of this tetrapeptide and heparin or dextran sulfate (but not chondroitin sulfate or hyaluronic acid) were additive. Nerve growth factor (NGF) pretreatment increased the percentage of PC12 cells adherent to all substrates and reduced the GAG inhibition of adhesion. PC12 cells previously treated with NGF to induce morphologic differentiation will rapidly re-extend neurites when plated on all four substrates. On fibronectin and poly-L-lysine-coated dishes this neurite growth is inhibited by added heparin and dextran sulfate, while on laminin it is not. Neurite formation on fibronectin-coated dishes was also inhibited by low concentrations of fibronectin tetrapeptide. In summary, PC12 adhesion and neurite formation can be inhibited by sulfated GAGs on some substrates, including fibronectin, but not other substrates, suggesting that these cells have at least two independent molecular adhesion mechanisms.