Gene expression differences between colon and rectum tumors.
Gene expression differences between colon and rectum tumors.
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DOI：
10.1158/1078-0432.ccr-11-1570
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发表时间：
2011-12-01
期刊：
影响因子：
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通讯作者：
Moreno V
中科院分区：
文献类型：
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作者：
Sanz-Pamplona R;Cordero D;Berenguer A;Lejbkowicz F;Rennert H;Salazar R;Biondo S;Sanjuan X;Pujana MA;Rozek L;Giordano TJ;Ben-Izhak O;Cohen HI;Trougouboff P;Bejhar J;Sova Y;Rennert G;Gruber SB;Moreno V
结直肠癌研究通常将结肠和直肠肿瘤作为一个共同的实体，尽管这一假设存在争议，并且在分子和流行病学水平上仅报道了微小的差异。我们基于结肠和直肠肿瘤的基因表达数据进行了一项分子研究，以评估这些癌症位点在转录组水平上的相似性程度。对来自3个独立研究的4个数据集的460个结肠肿瘤和100个直肠肿瘤进行了汇总分析。微卫星不稳定肿瘤被排除在外，因为已知这些肿瘤具有不同的表达谱，并且具有结肠近端优先位置。用线性模型评估表达差异，并通过多重比较调整确定显著基因。在近端结肠、远端结肠或直肠中出现的肿瘤在基因表达水平上存在微小差异。只有几个HOX基因被发现与肿瘤位置相关。结肠近端与远端差异大于结肠远端与直肠差异。微卫星稳定型结直肠癌在结肠或直肠肿瘤中没有显示出主要的转录组差异。观察到的微小但一致的差异主要是由HOX基因驱动的。这些结果可能对结直肠癌研究的设计和解释具有重要意义。
Colorectal cancer studies typically include both colon and rectum tumors as a common entity, though this assumption is controversial and only minor differences have been reported at the molecular and epidemiological level. We performed a molecular study based on gene expression data of tumors from colon and rectum to assess de degree of similarity between these cancer sites at transcriptomic level. A pooled analysis of 460 colon tumors and 100 rectum tumors from four datasets belonging to three independent studies was performed. Microsatellite instable tumors were excluded since these are known to have a different expression profile and have a preferential proximal colon location. Expression differences were assessed with linear models and significant genes were identified using adjustment for multiple comparisons. Minor differences at a gene expression level were found between tumors arising in the proximal colon, distal colon or rectum. Only several HOX genes were found to be associated with tumor location. More differences were found between proximal and distal colon that between distal colon and rectum. Microsatellite stable colorectal cancers do not show major transcriptomic differences for tumors arising in the colon or rectum. The small but consistent differences observed are largely driven by the HOX genes. These results may have important implications in the design and interpretation of studies in colorectal cancer.