Human osteoclasts express oxytocin receptor
Human osteoclasts express oxytocin receptor
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DOI：
10.1016/s0006-291x(02)02009-0
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发表时间：
2002-09-27
影响因子：
3.1
通讯作者：
Zallone, A
中科院分区：
文献类型：
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作者：
Colucci, S;Colaianni, G;Zallone, A
越来越多的证据表明下丘脑催产素有许多新的作用靶点，如调节食物平衡和某些情况下的瘦素分泌。考虑到瘦素是骨形成的有效抑制剂，并且在正常人成骨细胞中检测到催产素受体（OTR），我们研究了OTR是否由人破骨细胞（hOCs）表达以及激素对这些细胞的影响。在这里，我们通过免疫荧光和Western印迹分析证明了完全分化的hOCs及其前体（pOCs）的OTR表达。我们还表明，受体是功能性的，因为OT治疗诱导[Ca2+]（i）的增加，激素可能会影响破骨细胞生成，因为它增加了前破骨细胞的数量。(C)2002 Elsevier Science（美国）。All rights reserved.
Increasing evidences demonstrated many new targets for the hypothalamic hormone oxytocin, as the regulation of food balance and in some cases of leptin secretion. Considering that leptin is a potent inhibitor of bone formation and that oxytocin receptors (OTR) were detected in normal human osteoblasts, we investigated if OTR was expressed by human osteoclasts (hOCs) and the effect of the hormone on these cells. Here, we demonstrate by immunofluorescence and by Western blot analysis the expression of OTR by fully differentiated hOCs and by their precursors (pOCs). We also show that the receptor is functional, as OT treatment induces an increase of [Ca2+](i), and that the hormone may affect osteoclastogenesis, since it increases the number of pre-osteoclasts. (C) 2002 Elsevier Science (USA). All rights reserved.