Differentially expressed genes in nonsmall cell lung cancer:: expression profiling of cancer-related genes in squamous cell lung cancer
Differentially expressed genes in nonsmall cell lung cancer:: expression profiling of cancer-related genes in squamous cell lung cancer
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DOI：
10.1016/s0165-4608(03)00300-5
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发表时间：
2004-03-01
影响因子：
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通讯作者：
Wikman, H
中科院分区：
文献类型：
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作者：
Kettunen, E;Anttila, S;Wikman, H
对13例肺鳞癌（SCC）、13例非小细胞肺癌（NSCLC）腺癌（AC）和13例正常肺组织中癌相关基因的表达进行了分析。cDNA阵列用于筛选基因表达水平，并使用实时逆转录-聚合酶链反应（RT-PCR）验证阵列结果。在25个最上调和25个最下调的基因中，39%在SCC和AC中是共同的。在这些基因中，DSP HMGA 1（别名HMGIY）、TIMP 1、MIF、CCNB 1、TN、MMP 11和MMP 12上调，COPEB（别名CPBP）、TYROBP、贝内、BMPR 2、SOCS 3、TIMP 3、CAVI和CAV 2下调。与AC和正常肺相比，SCC中来自不同蛋白质家族（细胞角蛋白和半桥粒蛋白）的几个基因的表达水平显著增加。此外，在SCC中过表达的几个基因，如HMGA 1、CDK 4、IGFBP 3、MMP 9、MMP 11、MMP 12和MMP 14，落入不同的染色体位点，我们已经基于比较基因组杂交数据将其检测为获得的区域。我们的研究揭示了参与NSCLC的新候选基因。(C)2004年爱思唯尔公司All rights reserved.
The expression patterns of cancer-related genes in 13 cases of squamous cell lung cancer (SCC) were characterized and compared with those in normal lung tissue and 13 adenocarcinomas (AC), the other major type of nonsmall cell lung cancer (NSCLC). cDNA array was used to screen the gene expression levels and the array results were verified using a real-time reverse-transcriptase-polymerase chain reaction (RT-PCR). Thirty-nine percent of the 25 most upregulated and the 25 most downregulated genes were common to SCC and AC. Of these genes, DSP HMGA1 (alias HMGIY), TIMP1, MIF, CCNB1, TN, MMP11, and MMP12 were upregulated and COPEB (alias CPBP), TYROBP, BENE, BMPR2, SOCS3, TIMP3, CAVI, and CAV2 were downregulated. The expression levels of several genes from distinct protein families (cytokeratins and hemidesmosomal proteins) were markedly increased in SCC compared with AC and normal lung. In addition, several genes, overexpressed in SCC, such as HMGA1, CDK4, IGFBP3, MMP9, MMP11, MMP12, and MMP14, fell into distinct chromosomal loci, which we have detected as gained regions on the basis of comparative genomic hybridization data. Our study revealed new candidate genes involved in NSCLC. (C) 2004 Elsevier Inc. All rights reserved.