Identification of nutrient and physical seed trait QTL in the model legume Lotus japonicus
Identification of nutrient and physical seed trait QTL in the model legume Lotus japonicus
复制标题
DOI：
10.1139/g09-039
复制
发表时间：
2009-08-01
期刊：
影响因子：
3.1
通讯作者：
Grusak, Michael A.
中科院分区：
文献类型：
--
作者：
Klein, Melinda A.;Grusak, Michael A.
豆类种子有潜力为人类饮食提供重要的必需微量营养素。为探明种子营养密度的遗传基础，对模式豆科植物百脉根的Miyakojima MG-20 × Gifu B-129重组自交系群体进行了数量性状位点（QTL）分析。该种群于2006年和2007年在温室条件下种植种子。收集种子钙（Ca）、铜（Cu）、铁（Fe）、钾（K）、镁（Mg）、锰（Mn）、磷（P）、硫（S）和锌（Zn）浓度和含量的表型数据。物理种子性状（平均种子质量和种子荚分配值）的数据也被收集。基于这些表型数据，QTL分析确定了与55个不同分子标记连锁的103个QTL。在这一重组自交群体中发现的种子营养和物理性状的超亲分离表明，新的等位基因组合可用于农艺性状的改良。QTL共定位也被看到，这表明共同的运输过程可能有助于种子养分负荷。鉴定种子矿物质密度的基因座是鉴定遗传因素的重要第一步，因此，也是鉴定发育种子中矿物质分布的生理过程的重要第一步。长期的研究工作将侧重于通过相关作物豆类的直系同源物鉴定来促进农艺育种工作。
Legume seeds have the potential to provide a significant portion of essential micronutrients to the human diet. To identify the genetic basis for seed nutrient density, quantitative trait locus (QTL) analysis was conducted with the Miyakojima MG-20 x Gifu B-129 recombinant inbred population from the model legume Lotus japonicus. This population was grown to seed under greenhouse conditions in 2006 and 2007. Phenotypic data were collected for seed calcium (Ca), copper (Cu), iron (Fe), potassium (K), magnesium (Mg), manganese (Mn), phosphorus (P), sulfur (S), and zinc (Zn) concentrations and content. Data for physical seed traits (average seed mass and seed-pod allocation values) were also collected. Based on these phenotypic data, QTL analyses identified 103 QTL linked to 55 different molecular markers. Transgressive segregation, identified within this recombinant inbred population for both seed nutrient and physical traits, suggests new allelic combinations are available for agronomic trait improvement. QTL co-localization was also seen, suggesting that common transport processes might contribute to seed nutrient loading. Identification of loci involved in seed mineral density can be an important first step in identifying the genetic factors and, consequently, the physiological processes involved in mineral distribution to developing seeds. Longer term research efforts will focus on facilitating agronomic breeding efforts through ortholog identification in related crop legumes.