TRIM36 interacts with the kinetochore protein CENP-H and delays cell cycle progression
TRIM36 interacts with the kinetochore protein CENP-H and delays cell cycle progression
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DOI：
10.1016/j.bbrc.2009.02.059
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发表时间：
2009-04-10
影响因子：
3.1
通讯作者：
Hatakeyama, Shigetsugu
中科院分区：
文献类型：
--
作者：
Miyajima, Naoto;Maruyama, Satoru;Hatakeyama, Shigetsugu
tripartite motif-containing protein （TRIM）家族是由RING finger、B-box和coil -coil motif组成的共同结构域结构定义的。TRIM家族蛋白参与了广泛的生物过程，它们的改变会导致多种病理状况，如遗传病、病毒感染和癌症的发展。本研究通过酵母双杂交筛选发现TRIM36具有泛素连接酶活性，并与着丝点蛋白之一的着丝粒蛋白-h相互作用。我们还通过免疫荧光分析发现TRIM36与微管蛋白之一α -微管蛋白共定位。此外，我们发现TRIM36的过表达会减慢细胞周期并减弱细胞生长。这些结果表明TRIM36可能与染色体分离有关，过量的TRIM36可能导致染色体不稳定。(C) 2009爱思唯尔公司版权所有。
The tripartite motif-containing protein (TRIM) family is defined by the presence of a common domain structure composed of a RING finger, a B-box, and a coiled-coil motif. TRIM family proteins are involved in a broad range of biological processes and, consistently, their alterations result in diverse pathological conditions Such as genetic diseases, viral infection, and cancer development. In this Study, we found by using yeast two-hybrid screening that TRIM36 has a ubiquitin ligase activity and interacts with centromere protein-H, one of the kinetochore proteins. We also found by immunofluorescence analysis that TRIM36 colocalizes with alpha-tubulin, One of the microtubule proteins. Moreover, we found that overexpression of TRIM36 decelerates the cell cycle and attenuates cell growth. These results indicate that TRIM36 is potentially associated with chromosome segregation and that an excess of TRIM36 May Cause chromosomal instability. (C) 2009 Elsevier Inc. All rights reserved.