FORMATION AND ACTIVATION OF A CYCLIN E-CDK2 COMPLEX DURING THE G(1)-PHASE OF THE HUMAN CELL-CYCLE
FORMATION AND ACTIVATION OF A CYCLIN E-CDK2 COMPLEX DURING THE G(1)-PHASE OF THE HUMAN CELL-CYCLE
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DOI：
10.1126/science.1388288
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发表时间：
1992-09-18
期刊：
影响因子：
56.9
通讯作者：
ROBERTS, JM
中科院分区：
文献类型：
--
作者：
KOFF, A;GIORDANO, A;ROBERTS, JM
人类细胞周期蛋白E最初是根据其在发芽酵母中作为G1周期蛋白的功能而被发现的，与人类细胞中一种细胞周期调节的蛋白激酶有关。细胞周期蛋白E相关激酶活性在细胞周期蛋白A出现前的G1期达到峰值，在分化或血清撤除后退出细胞周期时减弱。人细胞中主要的细胞周期蛋白E相关蛋白是CDK2(细胞周期蛋白依赖性蛋白激酶2)。Cyclin E蛋白和Cyclin E-CDK2复合体在G1细胞中的丰度最高。这些结果进一步证明，在所有真核生物中，细胞周期蛋白-CDK复合体的组装是控制G1期细胞增殖的生化途径中的重要一步。
Human cyclin E, originally identified on the basis of its ability to function as a G1 cyclin in budding yeast, associated with a cell cycle-regulated protein kinase in human cells. The cyclin E-associated kinase activity peaked during G1, before the appearance of cyclin A, and was diminished during exit from the cell cycle after differentiation or serum withdrawal. The major cyclin E-associated kinase in human cells was Cdk2 (cyclin-dependent kinase 2). The abundance of the cyclin E protein and the cyclin E-Cdk2 complex was maximal in G1 cells. These results provide further evidence that in all eukaryotes assembly of a cyclin-Cdk complex is an important step in the biochemical pathway that controls cell proliferation during G1.