Construction and analysis of protein-protein interaction networks.
Construction and analysis of protein-protein interaction networks.
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DOI：
10.1186/1759-4499-2-2
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发表时间：
2010-02-15
期刊：
影响因子：
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通讯作者：
Raman K
中科院分区：
文献类型：
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作者：
Raman K
蛋白质-蛋白质相互作用形成了绝大多数细胞事件的基础，包括信号转导和转录调节。现在人们理解，细胞大分子之间相互作用的研究是理解生物系统的基础。蛋白质之间的相互作用已经通过许多高通量实验进行了研究，并且还通过利用过去十年中产生的大量序列数据的一系列计算方法进行了预测。在这篇综述中，我讨论了一些重要的计算方法预测蛋白质之间的功能联系。然后，我简要概述了一些对蛋白质相互作用研究有用的数据库和工具。我还介绍了网络理论，然后讨论了常用的参数在分析网络，重要的网络拓扑结构，以及方法来确定重要的网络组件，基于扰动。
Protein–protein interactions form the basis for a vast majority of cellular events, including signal transduction and transcriptional regulation. It is now understood that the study of interactions between cellular macromolecules is fundamental to the understanding of biological systems. Interactions between proteins have been studied through a number of high-throughput experiments and have also been predicted through an array of computational methods that leverage the vast amount of sequence data generated in the last decade. In this review, I discuss some of the important computational methods for the prediction of functional linkages between proteins. I then give a brief overview of some of the databases and tools that are useful for a study of protein–protein interactions. I also present an introduction to network theory, followed by a discussion of the parameters commonly used in analysing networks, important network topologies, as well as methods to identify important network components, based on perturbations.