Role of protein conformation and aggregation in pumping water in and out of a cell
Role of protein conformation and aggregation in pumping water in and out of a cell
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蛋白质构象和聚集在将水泵入和泵出细胞中的作用
DOI：
10.1016/j.cellbi.2005.09.010
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发表时间：
2006
影响因子：
3.9
通讯作者：
Gary D. Fullerton
中科院分区：
文献类型：
--
作者：
Ivan L. Cameron;K. Kanal;Gary D. Fullerton
使用含有BSA溶液的透析盒模拟被动体内条件，以评估蛋白质构象和聚集对细胞含水量的影响。将试剂盒悬浮在葡聚糖溶液中，以提供模拟血浆的一系列固定渗透应力值。将系统置于振荡器上24小时以达到平衡。四种手法;改变pH、共溶质盐浓度（例如NaCl）、温度退火和脲浓度变性剂以产生众所周知的BSA构象操纵。观察到细胞含水量从+14%变化到约-13%，蛋白质构象和聚集发生变化。研究结果表明，蛋白质构象和聚集的变化将水泵入和泵出细胞，以保持与蛋白质构象水合参数相匹配的平衡%水含量。这一概念补充了现有的细胞体积调节理论。
Dialysis cassettes containing BSA solutions were used to simulate passive in vivo conditions to assess the effect of protein conformation and aggregation on cell water content. The cassettes were suspended in dextran solutions to provide a range of fixed osmotic stress values simulating blood plasma. The system was placed on a shaker for 24 h to attain equilibrium. Four manipulation methods; pH, cosolute salt concentration, e.g. NaCl, temperature annealing and urea concentration denaturant were varied to produce well‐known manipulations of BSA conformation. It was observed that the cell water content varied from +14% to about −13% with changes in protein conformation and aggregation. The findings demonstrate that a change in protein conformation and aggregation, pumps water in and out of a cell to maintain equilibrium % water content matching the protein conformational hydration parameter. This concept supplements existing theories on cell volume regulation.
胃H,K-ATP酶的机制和结构。
影响因子：
18.2
作者：
Rabon,EC;Reuben,MA
通讯作者：
Reuben,MA