Study of urinary steroid hormone disorders: difference between hepatocellular carcinoma in early stage and cirrhosis
Study of urinary steroid hormone disorders: difference between hepatocellular carcinoma in early stage and cirrhosis
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尿类固醇激素紊乱研究：早期肝细胞癌与肝硬化的区别
DOI：
10.1007/s00216-014-7843-3
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发表时间：
2014-07-01
影响因子：
4.3
通讯作者：
Xu, Guowang
中科院分区：
文献类型：
--
作者：
Dai, Weidong;Yin, Peiyuan;Xu, Guowang
肝细胞癌（HCC）是世界上第五大常见癌症。从其他肝病如肝硬化中发现早期HCC的新生物标志物具有很大的临床益处。本研究采用液相色谱-质谱联用技术结合Logistic回归分析的类固醇激素代谢组学方法，对早期肝癌患者的类固醇激素代谢紊乱进行研究，筛选早期肝癌患者尿中潜在的类固醇激素生物标志物。在健康对照组、肝硬化患者和早期肝细胞癌患者中检测并定量了36种尿类固醇激素。热图分析和多元统计分析表明，在早期和早期肝癌患者中，类固醇激素网络严重紊乱，尿类固醇激素模式整体下降。Logistic回归分析显示，一组两种尿类固醇激素（肾上腺素和别四氢皮质醇）显示出良好的诊断能力，区分早期肝癌与肝硬化的曲线下面积（AUC）= 0.938的受试者工作特征（ROC）分析。这些结果有助于克服甲胎蛋白鉴别早期肝癌和肝硬化的敏感性和特异性较低的缺点。我们的工作表明，类固醇激素代谢组学是一种很有前途的生物标志物研究工具，用于早期肝癌的生物标志物研究。图类固醇激素代谢组学定量用于早期肝癌类固醇激素紊乱的研究和生物标志物的发现
Hepatocellular carcinoma (HCC) is the fifth most common cancer in the world. Discovery of novel biomarkers for early HCC from other liver diseases such as cirrhosis is of great clinical benefit. In this study, a novel steroid hormone metabolomic method based on liquid chromatography–mass spectrometry combined with logistic regression analysis was applied to study the steroid hormone disorders and to screen potential urinary steroid hormone biomarkers of early HCC. Thirty-six urinary steroid hormones were detected and quantified in healthy controls, cirrhotic patients, and early HCC patients. Heat map analysis and multivariate statistical analysis suggested severe disorders of steroid hormone network and holistically decreased urinary steroid hormone pattern in cirrhotic and early HCC patients. Logistic regression analysis reveals that a panel of two urinary steroid hormones (epitestosterone and allotetrahydrocortisol) displayed excellent diagnostic capability for distinguishing early HCC from cirrhosis with area under the curve (AUC) = 0.938 of receiver operating characteristic (ROC) analysis. These results help to overcome the disadvantage of lower sensitivity and specificity of alpha-fetoprotein for distinguishing early HCC from cirrhosis. Our work shows that steroid hormone metabolomics is a promising biomarker tool for biomarker study of early HCC.FigureSteroid hormone metabolome quantification for steroid hormone disorders investigation and biomarkers discovery of hepatocellular carcinoma in early stage