Culture of Enzymatically Dispersed Anterior Pituitary Cells: Functional Validation of a Method
Culture of Enzymatically Dispersed Anterior Pituitary Cells: Functional Validation of a Method
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酶法分散的垂体前叶细胞培养：方法的功能验证
DOI：
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发表时间：
1972
期刊：
影响因子：
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通讯作者：
R. Guillemin
中科院分区：
文献类型：
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作者：
W. Vale;G. Grant;M. Amoss;R. Blackwell;R. Guillemin
描述了一种制备短期酶分散垂体细胞培养物的方法。它已用于测定下丘脑释放因子。细胞分泌TSH或LH和FSH的量分别与TRF或LRF的剂量直接相关。合成 TRF 以及合成或高度纯化的羊 LRF 的最小活性剂量约为10-10M。 TRF 或 LRF 受体的表观亲和力常数约为10-9M。通常观察到 TSH 或 LH 的分泌率因 TRF 或 LRF 最高水平而增加 8 至 20 倍。尽管分泌反应的强度和细胞内激素含量随着培养时间的推移而下降，但培养细胞对TRF和LRF的敏感性可维持2周以上。长期施用 TRF 或 LRF 会消耗细胞内激素水平，同时增加培养物（组织和体液）中的总 TSH 或 LH 含量。正如在其他体外系统中观察到的那样，高培养基钾……
A method for preparing short—term enzymatically dispersed pituitary cell cultures is described. It has been used to assay hypothalamic releasing factors. The cells secrete TSH or LH and FSH in amounts directly related to the dose of TRF or LRF respectively. The minimal active doses of synthetic TRF and either synthetic or highly purified ovine LRF is ca. 10-10M. Apparent affinity constants for TRF or LRF receptors are ca. 10-9M. Enhanced secretion rates of TSH or LH of 8– to 20–fold in response to maximal levels of TRF or LRF are routinely observed. The sensitivity of the cultured cells to TRF and LRF is maintained for more than 2 weeks in culture although the magnitude of the secretory response and the intracellular hormone content declines with time in culture. Chronic administration of TRF or LRF depletes the intracellular hormone levels while simultaneously increasing the total TSH or LH content in the culture (tissue and fluid). As has been observed with other in vitro systems, high medium potassium,...