Pleiotropic effects of RsmA and RsmE proteins inPseudomonas fluorescens2P24
Pleiotropic effects of RsmA and RsmE proteins inPseudomonas fluorescens2P24
复制标题
RsmA和RsmE蛋白在荧光假单胞菌2P24中的多效性作用
DOI：
10.1186/s12866-020-01880-x
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发表时间：
2020-07-02
期刊：
影响因子：
4.2
通讯作者：
Zhang, Li-Qun
中科院分区：
文献类型：
--
作者：
Zhang, Yang;Zhang, Bo;Zhang, Li-Qun
背景荧光假单胞菌2P24是一种根际细菌，能产生2，4-二乙酰基间苯三酚(2，4-DAPG)作为抑制土传植物病害的决定性次生代谢产物。2，4-DAPG的生物合成受到RSMA家族蛋白RSMA和RSME的严格调控。然而，rsmA和rsmE基因的突变都会导致细菌生长减少。结果在本研究中，2，4-DAPG的过量产生影响了菌株2P24的生长。在双突变体中，当phlD基因缺失或编码2，4-DAPG水解酶的phlG基因过表达时，这种生长延迟可以部分逆转。对thersmA rsmE双突变体的RNA-Seq分析表明，很大一部分Thep.荧光基因组受RSMA家族蛋白调控。这些基因参与了2，4-DAPG的产生、细胞运动、碳代谢和第六型分泌系统的调节。结论RSMA和RSME是植物相关菌株2P24生态适合度相关基因的重要调节者，其调控细胞内2，4-DAPG浓度的机制较为复杂。
Background Pseudomonas fluorescens2P24 is a rhizosphere bacterium that produces 2,4-diacetyphloroglucinol (2,4-DAPG) as the decisive secondary metabolite to suppress soilborne plant diseases. The biosynthesis of 2,4-DAPG is strictly regulated by the RsmA family proteins RsmA and RsmE. However, mutation of both ofrsmAandrsmEgenes results in reduced bacterial growth. Results In this study, we showed that overproduction of 2,4-DAPG in thersmA rsmEdouble mutant influenced the growth of strain 2P24. This delay of growth could be partially reversal when thephlDgene was deleted or overexpression of thephlGgene encoding the 2,4-DAPG hydrolase in thersmA rsmEdouble mutant. RNA-seq analysis of thersmA rsmEdouble mutant revealed that a substantial portion of theP. fluorescensgenome was regulated by RsmA family proteins. These genes are involved in the regulation of 2,4-DAPG production, cell motility, carbon metabolism, and type six secretion system. Conclusions These results suggest that RsmA and RsmE are the important regulators of genes involved in the plant-associated strain 2P24 ecologic fitness and operate a sophisticated mechanism for fine-tuning the concentration of 2,4-DAPG in the cells.