Changes of diet and dominant intestinal microbes in farmland frogs.
Changes of diet and dominant intestinal microbes in farmland frogs.
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DOI：
10.1186/s12866-016-0660-4
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发表时间：
2016-03-10
期刊：
影响因子：
4.2
通讯作者：
Liao PC
中科院分区：
文献类型：
--
作者：
Chang CW;Huang BH;Lin SM;Huang CL;Liao PC
农业活动不可避免地造成对自然生境的人为干扰。农田野生动物的食物和肠道微生物区系可能会因为农业生态系统内食物网的变化而改变。在这项工作中，我们比较了自然和农田生境中淡色青蛙的饮食和肠道微生物区系，以了解习俗养殖如何影响体内微生物生态系统的健康。栖息于自然栖息地和农田栖息地的青蛙在胃内容物的出现、丰度和被捕食种类的数量上存在显著差异。此外，肠道微生物群落的丰富度、物种丰富度和α多样性的差异也具有统计学意义。微生物组成，特别是肠道中优势微生物的组成表明，土地利用方式可能是影响肠道微生物群落组成的因素之一。虽然在青蛙肠道中发现的前三个优势微生物门拟杆菌、拟杆菌和变形杆菌在生境中被归类为通用菌，但在自然环境中最占优势的肠道细菌门被农田蛙中的微生物门所取代。农田蛙肠道微生物丰富度的增加主要是由变形杆菌、放线杆菌、酸杆菌和植物真菌等多种微生物所贡献，这不仅反映了外部生态系统的改变可能导致微生物群落组成的变化，而且表明与疾病相关的微生物入侵的风险更高。这项研究表明，风俗养殖等人为活动不仅影响了青蛙的食物资源，还影响了野生动物的健康和体内微生物生态系统。本文的在线版本(doi：10.1186/s12866-0160660-4)包含补充材料，授权用户可以使用。
Agricultural activities inevitably result in anthropogenic interference with natural habitats. The diet and the gut microbiota of farmland wildlife can be altered due to the changes in food webs within agricultural ecosystems. In this work, we compared the diet and intestinal microbiota of the frog Fejervarya limnocharis in natural and farmland habitats in order to understand how custom farming affects the health of in vivo microbial ecosystems. The occurrence, abundance, and the numbers of prey categories of stomach content were significantly different between the frogs inhabiting natural and farmland habitats. In addition, differences in the abundance, species richness, and alpha-diversity of intestinal microbial communities were also statistically significant. The microbial composition, and particularly the composition of dominant microbes living in intestines, indicated that the land use practices might be one of factors affecting the gut microbial community composition. Although the first three dominant microbial phyla Bacteroidetes, Firmicutes, and Proteobacteria found in the intestines of frogs were classified as generalists among habitats, the most dominant gut bacterial phylum Bacteroidetes in natural environments was replaced by the microbial phylum Firmicutes in farmland frogs. Increased intestinal microbial richness of the farmland frogs, which is mostly contributed by numerous microbial species of Proteobacteria, Actinobacteria, Acidobacteria, and Planctomycetes, not only reflects the possible shifts in microbial community composition through the alteration of external ecosystem, but also indicates the higher risk of invasion by disease-related microbes. This study indicates that anthropogenic activities, such as the custom farming, have not only affected the food resources of frogs, but also influenced the health and in vivo microbial ecosystem of wildlife. The online version of this article (doi:10.1186/s12866-016-0660-4) contains supplementary material, which is available to authorized users.