Combined effects of polyfluorinated and perfluorinated compounds on primary cultured hepatocytes from rare minnow (Gobiocypris rarus) using toxicogenomic analysis
Combined effects of polyfluorinated and perfluorinated compounds on primary cultured hepatocytes from rare minnow (Gobiocypris rarus) using toxicogenomic analysis
复制标题
使用毒物基因组分析，多氟和全氟化合物对稀有鲦鱼（Gobiocypris rarus）原代培养肝细胞的综合影响
DOI：
10.1016/j.aquatox.2009.07.020
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发表时间：
2009-10-19
影响因子：
4.5
通讯作者：
Dai, Jiayin
中科院分区：
文献类型：
--
作者：
Wei, Yanhong;Shi, Xiongjie;Dai, Jiayin
多氟和全氟化合物(PFC)用于许多商业产品，并在环境以及人类和野生动物的血液中普遍检测到。为了评估PFC在混合物中所引起的联合效应，使用定制的基因表达谱芯片检测了6种不同的PFC(全氟辛酸、全氟农酸、全氟十一酸、全氟碘正十二酸、全氟辛烷磺酸和8：2氟调质醇)和4种配方的PFC混合物对稀有小鱼原代培养肝细胞的影响。混合物以及单独的化合物一致地调节着特定的基因集，这表明这些保守的基因可能在PFC介导的毒性中发挥核心作用。具体地说，在这项研究中，确定了一些受混合物调控的基因，这些基因不受接触任何单一成分的影响。这些基因涉及多种生物学功能和过程，包括脂肪酸代谢和运输、异体代谢、免疫反应和氧化应激。在全氟辛烷磺酸和全氟辛烷磺酸显性混合暴露组中，超过80%的改变基因属于相同的基因组，而单一全氟辛烷磺酸和全氟辛烷磺酸暴露的基因表达谱不同。这项工作有助于开发联合毒性评估中的毒性基因组学方法，并允许全面了解全氟碳化合物混合物在多种环境基质中的联合作用。(C)2009爱思唯尔B.V.保留所有权利。
Polyfluorinated and perfluorinated compounds (PFCs) are used in numerous commercial products and have been ubiquitously detected in the environment as well as in the blood of humans and wildlife. To assess the combined effects caused by PFCs in mixtures, gene expression profiles were generated using a custom cDNA microarray to detect changes in primary cultured hepatocytes of rare minnows exposed to six individual PFCs (perfluorooctanoic acid, perfluorononanoic acid, perfluorodecanoic acid, perfluorododecanoic acid, perfluorooctane sulfonate, and 8:2 fluorotelomer alcohol) and four formulations of the PFCs mixtures. Mixtures as well as individual compounds consistently regulated a particular gene set, which suggests that these conserved genes may play a central role in the toxicity mediated by PFCs. Specifically, a number of genes regulated by the mixtures were identified in this study, which were not affected by exposure to any single component. These genes are implicated in multiple biological functions and processes, including fatty acid metabolism and transport, xenobiotic metabolism, immune responses, and oxidative stress. More than 80% of the altered genes in the PFOA- and PFOS-dominant mixture groups were of the same gene set, while the gene expression profiles from single PFOA and PFOS exposures were not as similar. This work contributes to the development of toxicogenomic approaches in combined toxicity assessment and allows for comprehensive insights into the combined action of PFCs mixtures in multiple environmental matrices. (C) 2009 Elsevier B.V. All rights reserved.