RNA interface-mediated reduction in GLUT1 inhibits serum-induced glucose transport in primary human skeletal muscle cells
RNA interface-mediated reduction in GLUT1 inhibits serum-induced glucose transport in primary human skeletal muscle cells
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DOI：
10.1016/s0006-291x(03)01124-0
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发表时间：
2003-07-18
影响因子：
3.1
通讯作者：
Krook, A
中科院分区：
文献类型：
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作者：
Al-Khalili, L;Cartee, GD;Krook, A
利用RNA干扰(RNAi)，我们特异性地下调了分化的人骨骼肌管培养物中的蛋白质表达。血清刺激肌管可增加葡萄糖摄取。利用灵敏的光标记技术，我们证明了葡萄糖摄取量的增加伴随着细胞表面葡萄糖转运体(GLUT)1含量的增加。利用RNAi，我们特异性地降低了GLUT1的mRNA和蛋白的表达，导致抑制血清介导的葡萄糖转运的增加。因此，我们证明了RNAi在人类原代分化细胞系统中的作用，并应用这一方法学证明了血清介导的人类骨骼肌细胞葡萄糖转运的增加依赖于GLUT1。(C)2003年埃尔塞维尔科学公司(美国)。版权所有。
Using RNA interference (RNAi), we specifically down-regulate protein expression in differentiated human skeletal myotube cultures. Serum stimulation of myotubes increases glucose uptake. Using a sensitive photolabeling technique, we demonstrate that this increase in glucose uptake is accompanied by increased cell-surface content of glucose transporter (GLUT) 1. Using RNAi, we specifically reduce GLUT1 mRNA and protein expression, leading to inhibition of serum-mediated increase in glucose transport. Thus, we demonstrate the utility of RNAi in a primary human differentiated cell system, and apply this methodology to demonstrate that serum-mediated increase in glucose transport in human skeletal muscle cells is dependent on GLUT1. (C) 2003 Elsevier Science (USA). All rights reserved.