Differential effects of extracellular matrix on secretion of prolactin and growth hormone by rat pituitary tumour cells in vitro.
Differential effects of extracellular matrix on secretion of prolactin and growth hormone by rat pituitary tumour cells in vitro.
复制标题
细胞外基质对体外大鼠垂体肿瘤细胞分泌催乳素和生长激素的不同影响。
DOI：
--
复制
发表时间：
1985
期刊：
影响因子：
--
通讯作者：
J. Jenkins
中科院分区：
文献类型：
--
作者：
R. A. Prysor;J. Silverlight;J. Jenkins
将两种分泌催乳素（Prl）和生长激素（GH）的大鼠垂体肿瘤细胞分别培养在塑料培养皿和涂有牛角膜内皮细胞外基质（ECM）的表面上。ECM的存在导致Prl升高而GH降低。在一个细胞系中，ECM增加了Prl对促甲状腺激素释放激素（TRH）的反应。当使用ECM时，培养物的传播速度增加，细胞蛋白质和DNA合成增加，细胞形态发生变化。有人认为，这些观察结果可以通过ECM对血清中存在的生长因子的致敏作用来解释。
Two types of rat pituitary tumour cells secreting both prolactin (Prl) and growth hormone (GH) were cultured in vitro either on plastic dishes or on surfaces coated with an extracellular matrix (ECM) derived from bovine corneal endothelium. The presence of ECM caused an increase in Prl but a decrease in GH. On one cell line the Prl response to thyrotrophin releasing hormone (TRH) was increased by ECM. There was an increase in the rate of spread of the cultures, an increase in cell protein, and DNA synthesis and a change in cell morphology when ECM was used. It is suggested that these observations can be explained by a sensitizing action of ECM to growth factors present in serum.